Reviewed, UniProtKB/Swiss-Prot A8A0T8 (ASTD_ECOHS)

Names and originHide

Protein names

Recommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase
EC=1.2.1.71

Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	EcHS_A1829

Organism

Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]

Taxonomic identifier

331112 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributesHide

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

OntologiesHide

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000065755
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	277	1	By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	A8A0T8-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 64AEF9CAC0E7DA5F	FASTA	492	52,988
	10 20 30 40 50 60 MTLWINGDWI TGQGASRVKR NPVSGEVLWQ GNDAGAAQVE QACRAARAAF PRWARLSLAE 70 80 90 100 110 120 RQVVVERFAG LLESNKAELT AIIARETGKP RWEAATEVTA MINKIAISIK AYHVRTGEQR 130 140 150 160 170 180 SEMPDGAASL RHRPHGVLAV FGPYNFPGHL PNGHIVPALL AGNTIIFKPS ELTPWSGEAV 190 200 210 220 230 240 MRLWQQAGLP PGVLNLVQGG RETGQALSAL EDLDGLLFTG SANTGYQLHR QLSGQPEKIL 250 260 270 280 290 300 ALEMGGNNPL IIDEVADIDA AVHLTIQSAF VTAGQRCTCA RRLLLKSGAQ GDAFLARLVA 310 320 330 340 350 360 VSQRLTPGNW DDEPQPFIGG LISEQAAQQV VTAWQQLEAM GGRTLLAPRL LQSETSLLTP 370 380 390 400 410 420 GIIEMTGVAG VPDEEVFGPL LRVWRYDSFE EAILMANNTR FGLSCGLVSP EREKFDQLLL 430 440 450 460 470 480 EARAGIVNWN KPLTGAASTA PFGGIGASGN HRPSAWYAAD YCAWPMASLE SDSLTLPATL 490 NPGLDFSDEV VR

ReferencesHide

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	CP000802 Genomic DNA. Translation: ABV06142.1.
RefSeq	YP_001458525.1.
3D structure databases
SMR	A8A0T8. Positions 3-462.
ModBase	Search...
Protein-protein interaction databases
STRING	A8A0T8.
Genome annotation databases
EnsemblBacteria	EBESCT00000054896; EBESCP00000052881; EBESCG00000053944.
GeneID	5591866.
GenomeReviews	Gene locus EcHS_A1829 in contig CP000802_GR.
KEGG	ecx:EcHS_A1829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	KAYHART.
ProtClustDB	PRK09457.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

ASTD_ECOHS

Accession

Primary (citable) accession number: A8A0T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	June 15, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)