A7ZWZ7 (MHPD_ECOHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-keto-4-pentenoate hydratase
EC=4.2.1.80

Alternative name(s):
2-hydroxypentadienoic acid hydratase

Gene names

Name:	mhpD
Ordered Locus Names:	EcHS_A0414

Organism

Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]

Taxonomic identifier

331112 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid By similarity. HAMAP-Rule MF_01655
Catalytic activity	4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O. HAMAP-Rule MF_01655
Cofactor	Divalent metal ions By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01655
Sequence similarities	Belongs to the hydratase/decarboxylase family. MhpD subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	3-phenylpropionate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-oxopent-4-enoate hydratase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	2-keto-4-pentenoate hydratase HAMAP-Rule MF_01655		PRO_0000337795

Sequences

	Sequence		Length	Mass (Da)
	A7ZWZ7 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 4E9C4719F16BA83B	FASTA	269	28,914
	10 20 30 40 50 60 MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYA IQHINVQYDV VQGRRVVGRK 70 80 90 100 110 120 VGLTHPKVQQ QLGVDQPDFG TLFADMCYGD NEIIPFSRVL QPRIEAEIAL VLNRDLPATD 130 140 150 160 170 180 ITFDELYNAI EWVLPALEVV GSRIRDWSIQ FVDTVADNAS CGVYVIGGPA QRPAGLDLKN 190 200 210 220 230 240 CAMKMTRNNE EVSSGRGSEC LGHPLNAAVW LARKMASLGE PLRAGDIILT GALGPMVAVN 250 260 AGDRFEAHIE GIGSVAATFS SAAPKGSLS

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000802 Genomic DNA. Translation: ABV04801.1.
RefSeq	YP_001457184.1. NC_009800.1.
3D structure databases
ProteinModelPortal	A7ZWZ7.
SMR	A7ZWZ7. Positions 1-261.
ModBase	Search...
Protein-protein interaction databases
STRING	331112.EcHS_A0414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV04801; ABV04801; EcHS_A0414.
GeneID	5595036.
KEGG	ecx:EcHS_A0414.
PATRIC	18310826. VBIEscCol77814_0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3971.
HOGENOM	HOG000179637.
KO	K02554.
OMA	REVTMRM.
ProtClustDB	PRK11342.
Enzyme and pathway databases
BioCyc	ECOL331112:GHHI-560-MONOMER.
UniPathway	UPA00714.
Family and domain databases
Gene3D	3.90.850.10. 1 hit.
HAMAP	MF_01655. MhpD.
InterPro	IPR002529. Fumarylacetoacetase_C. IPR011234. Fumarylacetoacetase_C-rel. IPR023793. Keto_pentenoate-hydratase. [Graphical view]
Pfam	PF01557. FAA_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56529. Fumarylacetoacetase_C-rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPD_ECOHS

Accession

Primary (citable) accession number: A7ZWZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program