A7ZTA0 (GHRB_ECO24)
Reviewed,
UniProtKB/Swiss-Prot
Last modified
August 10, 2010.
Version 23.
History...
Names and origin
| Protein names | Recommended name: Glyoxylate/hydroxypyruvate reductase B EC=1.1.1.79 EC=1.1.1.81 | ||||
| Gene names |
| ||||
| Organism | Escherichia coli O139:H28 (strain E24377A / ETEC) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 331111 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 324 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667 |
| Catalytic activity | Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01667 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_01667. |
| Sequence similarities | Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | NAD NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activityInferred from electronic annotation. Source: EC hydroxypyruvate reductase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 324 | 324 | Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667 | PRO_0000348387 | |||||
Sites | |||||||||
| Active site | 237 | 1 | By similarity | ||||||
| Active site | 266 | 1 | By similarity | ||||||
| Active site | 285 | 1 | Proton donor By similarity | ||||||
Sequences
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References
| [1] | "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000800 Genomic DNA. Translation: ABV17627.1. |
| RefSeq | YP_001465029.1. |
3D structure databases | |
| ProteinModelPortal | A7ZTA0. |
| SMR | A7ZTA0. Positions 1-323. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A7ZTA0. |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000019757; EBESCP00000018819; EBESCG00000018811. |
| GeneID | 5590663. |
| GenomeReviews | Gene locus EcE24377A_4049 in contig CP000800_GR. |
| KEGG | ecw:EcE24377A_4049. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1052. |
| HOGENOM | HBG731446. |
| OMA | WIKGAGL. |
| ProtClustDB | CLSK889638. |
Enzyme and pathway databases | |
| BioCyc | ECOL331111:ECE24377A_4049-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01667. 2-Hacid_dh_C_GhrB. [Tree] |
| InterPro | IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view] |
| PROSITE | PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GHRB_ECO24 | ||||||||
| Accession | Primary (citable) accession number: A7ZTA0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with


