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Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Removes maltotriose and maltotetraose chains that are attached by 1,6-alpha-linkage to the limit dextrin main chain, generating a debranched limit dextrin.UniRule annotation

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches with degrees of polymerization of three or four glucose residues in limit dextrin.UniRule annotation

Pathwayi: glycogen degradation

This protein is involved in the pathway glycogen degradation, which is part of Glycan degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei336NucleophileUniRule annotation1
Active sitei371Proton donorUniRule annotation1
Sitei443Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00165.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzymeUniRule annotation (EC:3.2.1.196UniRule annotation)
Alternative name(s):
Limit dextrin alpha-1,6-maltotetraose-hydrolaseUniRule annotation
Gene namesi
Name:glgXUniRule annotation
Ordered Locus Names:EcE24377A_3910
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001122 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000670981 – 657Glycogen debranching enzymeAdd BLAST657

Structurei

3D structure databases

ProteinModelPortaliA7ZSW4.
SMRiA7ZSW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000239197.
KOiK02438.
OMAiSEPWDCG.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
HAMAPiMF_01248. GlgX. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZSW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP
60 70 80 90 100
GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI
110 120 130 140 150
DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS
160 170 180 190 200
TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP
210 220 230 240 250
VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA
260 270 280 290 300
LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT
310 320 330 340 350
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD
360 370 380 390 400
APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA
410 420 430 440 450
RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC
460 470 480 490 500
VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL
510 520 530 540 550
TTLLLSQGTP MLLAGDEHGH SQYGNNNAYC QDNQLTWLDW SQASSGLTAF
560 570 580 590 600
TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ
610 620 630 640 650
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH

GLCVFQR
Length:657
Mass (Da):73,603
Last modified:October 23, 2007 - v1
Checksum:iBF8BFC1F72E80B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV16561.1.
RefSeqiWP_000192526.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV16561; ABV16561; EcE24377A_3910.
KEGGiecw:EcE24377A_3910.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLGX_ECO24
AccessioniPrimary (citable) accession number: A7ZSW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: July 5, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families