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A7ZSW4

- GLGX_ECO24

UniProt

A7ZSW4 - GLGX_ECO24

Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei336 – 3361NucleophileUniRule annotation
    Active sitei371 – 3711Proton donorUniRule annotation
    Sitei443 – 4431Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. glycogen debranching enzyme activity Source: UniProtKB-HAMAP
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciECOL331111:GH7P-3889-MONOMER.
    UniPathwayiUPA00165.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen debranching enzymeUniRule annotation (EC:3.2.1.-UniRule annotation)
    Alternative name(s):
    Glycogen operon protein GlgXUniRule annotation
    Gene namesi
    Name:glgXUniRule annotation
    Ordered Locus Names:EcE24377A_3910
    OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
    Taxonomic identifieri331111 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001122: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Glycogen debranching enzymePRO_1000067098Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi331111.EcE24377A_3910.

    Structurei

    3D structure databases

    ProteinModelPortaliA7ZSW4.
    SMRiA7ZSW4. Positions 2-657.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1523.
    HOGENOMiHOG000239197.
    KOiK02438.
    OMAiGKTNYWG.
    OrthoDBiEOG64BQ3X.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01248. GlgX.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR022844. Glycogen_debranch_bac.
    IPR011837. Glycogen_debranch_GlgX.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A7ZSW4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP    50
    GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI 100
    DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS 150
    TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP 200
    VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA 250
    LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT 300
    GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD 350
    APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA 400
    RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC 450
    VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL 500
    TTLLLSQGTP MLLAGDEHGH SQYGNNNAYC QDNQLTWLDW SQASSGLTAF 550
    TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ 600
    ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH 650
    GLCVFQR 657
    Length:657
    Mass (Da):73,603
    Last modified:October 23, 2007 - v1
    Checksum:iBF8BFC1F72E80B85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000800 Genomic DNA. Translation: ABV16561.1.
    RefSeqiWP_000192526.1. NC_009801.1.
    YP_001464893.1. NC_009801.1.

    Genome annotation databases

    EnsemblBacteriaiABV16561; ABV16561; EcE24377A_3910.
    GeneIDi5587748.
    KEGGiecw:EcE24377A_3910.
    PATRICi18297092. VBIEscCol31211_4151.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000800 Genomic DNA. Translation: ABV16561.1 .
    RefSeqi WP_000192526.1. NC_009801.1.
    YP_001464893.1. NC_009801.1.

    3D structure databases

    ProteinModelPortali A7ZSW4.
    SMRi A7ZSW4. Positions 2-657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331111.EcE24377A_3910.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV16561 ; ABV16561 ; EcE24377A_3910 .
    GeneIDi 5587748.
    KEGGi ecw:EcE24377A_3910.
    PATRICi 18297092. VBIEscCol31211_4151.

    Phylogenomic databases

    eggNOGi COG1523.
    HOGENOMi HOG000239197.
    KOi K02438.
    OMAi GKTNYWG.
    OrthoDBi EOG64BQ3X.

    Enzyme and pathway databases

    UniPathwayi UPA00165 .
    BioCyci ECOL331111:GH7P-3889-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01248. GlgX.
    InterProi IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR022844. Glycogen_debranch_bac.
    IPR011837. Glycogen_debranch_GlgX.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR02100. glgX_debranch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
      Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
      J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E24377A / ETEC.

    Entry informationi

    Entry nameiGLGX_ECO24
    AccessioniPrimary (citable) accession number: A7ZSW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3