A7ZSW4 (GLGX_ECO24) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen debranching enzyme
EC=3.2.1.-

Alternative name(s):
Glycogen operon protein GlgX

Gene names

Name:	glgX
Ordered Locus Names:	EcE24377A_3910

Organism

Escherichia coli O139:H28 (strain E24377A / ETEC) [Complete proteome] [HAMAP]

Taxonomic identifier

331111 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen By similarity. HAMAP-Rule MF_01248
Pathway	Glycan degradation; glycogen degradation. HAMAP-Rule MF_01248
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro glycogen debranching enzyme activity Inferred from electronic annotation. Source: HAMAP hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Glycogen debranching enzyme HAMAP-Rule MF_01248		PRO_1000067098
Sites
	Active site	336	1	Nucleophile By similarity
	Active site	371	1	Proton donor By similarity
	Site	443	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZSW4 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: BF8BFC1F72E80B85	FASTA	657	73,603
	10 20 30 40 50 60 MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP GHSGDIWHGY 70 80 90 100 110 120 LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI DGEFKDNPLL HAGHNEPDYR 130 140 150 160 170 180 DNAAIAPKCV VVVDHYDWED DAPPRTPWGS TIIYEAHVKG LTYLHPEIPV EIRGTYKALG 190 200 210 220 230 240 HPVMINYLKQ LGITALELLP VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA 250 260 270 280 290 300 LDEFRDAIKA LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT 310 320 330 340 350 360 GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD APLFTAIQNC 370 380 390 400 410 420 PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA RRFWLHYDLP LGAFAGRFAA 430 440 450 460 470 480 SSDVFKRNGR LPSAAINLVT AHDGFTLRDC VCFNHKHNEA NGEENRDGTN NNYSNNHGKE 490 500 510 520 530 540 GLGGSLDLVE RRRDSIHALL TTLLLSQGTP MLLAGDEHGH SQYGNNNAYC QDNQLTWLDW 550 560 570 580 590 600 SQASSGLTAF TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ 610 620 630 640 650 ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH GLCVFQR

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E24377A / ETEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000800 Genomic DNA. Translation: ABV16561.1.
RefSeq	YP_001464893.1. NC_009801.1.
3D structure databases
ProteinModelPortal	A7ZSW4.
SMR	A7ZSW4. Positions 2-657.
ModBase	Search...
Protein-protein interaction databases
STRING	331111.EcE24377A_3910.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV16561; ABV16561; EcE24377A_3910.
GeneID	5587748.
KEGG	ecw:EcE24377A_3910.
PATRIC	18297092. VBIEscCol31211_4151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1523.
HOGENOM	HOG000239197.
KO	K02438.
OMA	HPVMIAY.
ProtClustDB	PRK03705.
Enzyme and pathway databases
BioCyc	ECOL331111:GH7P-3926-MONOMER.
UniPathway	UPA00165.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_01248. GlgX.
InterPro	IPR015902. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR022844. Glycogen_debranch_bac. IPR011837. Glycogen_debranch_debranch. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF74. PTHR10357:SF74. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02100. glgX_debranch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGX_ECO24

Accession

Primary (citable) accession number: A7ZSW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program