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A7ZSW4

- GLGX_ECO24

UniProt

A7ZSW4 - GLGX_ECO24

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Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei336 – 3361NucleophileUniRule annotation
Active sitei371 – 3711Proton donorUniRule annotation
Sitei443 – 4431Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. glycogen debranching enzyme activity Source: UniProtKB-HAMAP
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciECOL331111:GH7P-3889-MONOMER.
UniPathwayiUPA00165.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzymeUniRule annotation (EC:3.2.1.-UniRule annotation)
Alternative name(s):
Glycogen operon protein GlgXUniRule annotation
Gene namesi
Name:glgXUniRule annotation
Ordered Locus Names:EcE24377A_3910
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001122: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Glycogen debranching enzymePRO_1000067098Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi331111.EcE24377A_3910.

Structurei

3D structure databases

ProteinModelPortaliA7ZSW4.
SMRiA7ZSW4. Positions 2-657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1523.
HOGENOMiHOG000239197.
KOiK02438.
OMAiGKTNYWG.
OrthoDBiEOG64BQ3X.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01248. GlgX.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZSW4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP
60 70 80 90 100
GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI
110 120 130 140 150
DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS
160 170 180 190 200
TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP
210 220 230 240 250
VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA
260 270 280 290 300
LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT
310 320 330 340 350
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD
360 370 380 390 400
APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA
410 420 430 440 450
RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC
460 470 480 490 500
VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL
510 520 530 540 550
TTLLLSQGTP MLLAGDEHGH SQYGNNNAYC QDNQLTWLDW SQASSGLTAF
560 570 580 590 600
TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ
610 620 630 640 650
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH

GLCVFQR
Length:657
Mass (Da):73,603
Last modified:October 23, 2007 - v1
Checksum:iBF8BFC1F72E80B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV16561.1.
RefSeqiWP_000192526.1. NC_009801.1.
YP_001464893.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV16561; ABV16561; EcE24377A_3910.
GeneIDi5587748.
KEGGiecw:EcE24377A_3910.
PATRICi18297092. VBIEscCol31211_4151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV16561.1 .
RefSeqi WP_000192526.1. NC_009801.1.
YP_001464893.1. NC_009801.1.

3D structure databases

ProteinModelPortali A7ZSW4.
SMRi A7ZSW4. Positions 2-657.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331111.EcE24377A_3910.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV16561 ; ABV16561 ; EcE24377A_3910 .
GeneIDi 5587748.
KEGGi ecw:EcE24377A_3910.
PATRICi 18297092. VBIEscCol31211_4151.

Phylogenomic databases

eggNOGi COG1523.
HOGENOMi HOG000239197.
KOi K02438.
OMAi GKTNYWG.
OrthoDBi EOG64BQ3X.

Enzyme and pathway databases

UniPathwayi UPA00165 .
BioCyci ECOL331111:GH7P-3889-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01248. GlgX.
InterProi IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR02100. glgX_debranch. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiGLGX_ECO24
AccessioniPrimary (citable) accession number: A7ZSW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3