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A7ZRK3

- A7ZRK3_ECO24

UniProt

A7ZRK3 - A7ZRK3_ECO24

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Protein
Malate synthase G
Gene
glcB, EcE24377A_3435
Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarityUniRule annotation
Binding sitei274 – 2741Acetyl-CoA By similarityUniRule annotation
Binding sitei311 – 3111Acetyl-CoA By similarityUniRule annotation
Active sitei338 – 3381Proton acceptor By similarityUniRule annotation
Binding sitei338 – 3381Glyoxylate By similarityUniRule annotation
Metal bindingi427 – 4271Magnesium By similarityUniRule annotation
Binding sitei427 – 4271Glyoxylate By similarityUniRule annotation
Metal bindingi455 – 4551Magnesium By similarityUniRule annotation
Binding sitei536 – 5361Acetyl-CoA; via carbonyl oxygen By similarityUniRule annotation
Active sitei631 – 6311Proton donor By similarityUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

AcyltransferaseImported, Transferase

Keywords - Biological processi

Glyoxylate bypassUniRule annotationSAAS annotations, Tricarboxylic acid cycleUniRule annotationSAAS annotations

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciECOL331111:GH7P-3416-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotationImported
Ordered Locus Names:EcE24377A_3435Imported
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)Imported
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001122: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH) By similarityUniRule annotation
Modified residuei688 – 6881Cysteine sulfenic acid (-SOH) By similarityUniRule annotation

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331111.EcE24377A_3435.

Structurei

3D structure databases

ProteinModelPortaliA7ZRK3.
SMRiA7ZRK3. Positions 1-723.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarityUniRule annotation
Regioni452 – 4554Glyoxylate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPANTLKM.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZRK3-1 [UniParc]FASTAAdd to Basket

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MSQTITQGRL RIDANFKRFV DEEVLPGTGQ DAAAFWRNFD EIVHDLAPEN    50
RQLLAERDRI QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV 100
ETTGIDSEIT SQAGPQLVVP AMNARYALNA ANARWGSLYD ALYGSDIIPQ 150
EGAMVSGYDP QRGEQVIAWV RRFLDESLPL ENGSYQDVVA FKVVDKQLRI 200
QLKNGKETTL RTPAQFVGYR GDAAALTCIL LKNNGLHIEL QIDANGRIGK 250
DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 300
KMEKNGRQIV RKLNDDRHYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW 350
DSEGNEIPEG ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA 400
FANKLFTRIE TMLGMAPNTL KMGIMDEERR TSLNLRSCIA QARNRVAFIN 450
TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP WIKAYERNNV LSGLFCGLRG 500
KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA ATLHALHYHQ 550
TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSVE EIQQELDNNV 600
QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI 650
LTKEQVQASL ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG 700
VKQPNGYTEP LLHAWRLREK ESH 723
Length:723
Mass (Da):80,519
Last modified:October 23, 2007 - v1
Checksum:i4B160E959727C3F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000800 Genomic DNA. Translation: ABV18902.1.
RefSeqiYP_001464432.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV18902; ABV18902; EcE24377A_3435.
GeneIDi5590624.
KEGGiecw:EcE24377A_3435.
PATRICi18296186. VBIEscCol31211_3708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000800 Genomic DNA. Translation: ABV18902.1 .
RefSeqi YP_001464432.1. NC_009801.1.

3D structure databases

ProteinModelPortali A7ZRK3.
SMRi A7ZRK3. Positions 1-723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331111.EcE24377A_3435.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV18902 ; ABV18902 ; EcE24377A_3435 .
GeneIDi 5590624.
KEGGi ecw:EcE24377A_3435.
PATRICi 18296186. VBIEscCol31211_3708.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi PANTLKM.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci ECOL331111:GH7P-3416-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiA7ZRK3_ECO24
AccessioniPrimary (citable) accession number: A7ZRK3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 23, 2007
Last sequence update: October 23, 2007
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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