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A7ZRK3

- A7ZRK3_ECO24

UniProt

A7ZRK3 - A7ZRK3_ECO24

Protein

Malate synthase G

Gene

glcB

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei274 – 2741Acetyl-CoAUniRule annotation
    Binding sitei311 – 3111Acetyl-CoAUniRule annotation
    Active sitei338 – 3381Proton acceptorUniRule annotation
    Binding sitei338 – 3381GlyoxylateUniRule annotation
    Metal bindingi427 – 4271MagnesiumUniRule annotation
    Binding sitei427 – 4271GlyoxylateUniRule annotation
    Metal bindingi455 – 4551MagnesiumUniRule annotation
    Binding sitei536 – 5361Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    AcyltransferaseImported, Transferase

    Keywords - Biological processi

    Glyoxylate bypassUniRule annotationSAAS annotation, Tricarboxylic acid cycleUniRule annotationSAAS annotation

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciECOL331111:GH7P-3416-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotationImported
    Ordered Locus Names:EcE24377A_3435Imported
    OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)Imported
    Taxonomic identifieri331111 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001122: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation
    Modified residuei688 – 6881Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    OxidationUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331111.EcE24377A_3435.

    Structurei

    3D structure databases

    ProteinModelPortaliA7ZRK3.
    SMRiA7ZRK3. Positions 1-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni452 – 4554Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiPANTLKM.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A7ZRK3-1 [UniParc]FASTAAdd to Basket

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    MSQTITQGRL RIDANFKRFV DEEVLPGTGQ DAAAFWRNFD EIVHDLAPEN    50
    RQLLAERDRI QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV 100
    ETTGIDSEIT SQAGPQLVVP AMNARYALNA ANARWGSLYD ALYGSDIIPQ 150
    EGAMVSGYDP QRGEQVIAWV RRFLDESLPL ENGSYQDVVA FKVVDKQLRI 200
    QLKNGKETTL RTPAQFVGYR GDAAALTCIL LKNNGLHIEL QIDANGRIGK 250
    DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 300
    KMEKNGRQIV RKLNDDRHYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW 350
    DSEGNEIPEG ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA 400
    FANKLFTRIE TMLGMAPNTL KMGIMDEERR TSLNLRSCIA QARNRVAFIN 450
    TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP WIKAYERNNV LSGLFCGLRG 500
    KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA ATLHALHYHQ 550
    TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSVE EIQQELDNNV 600
    QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI 650
    LTKEQVQASL ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG 700
    VKQPNGYTEP LLHAWRLREK ESH 723
    Length:723
    Mass (Da):80,519
    Last modified:October 23, 2007 - v1
    Checksum:i4B160E959727C3F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000800 Genomic DNA. Translation: ABV18902.1.
    RefSeqiYP_001464432.1. NC_009801.1.

    Genome annotation databases

    EnsemblBacteriaiABV18902; ABV18902; EcE24377A_3435.
    GeneIDi5590624.
    KEGGiecw:EcE24377A_3435.
    PATRICi18296186. VBIEscCol31211_3708.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000800 Genomic DNA. Translation: ABV18902.1 .
    RefSeqi YP_001464432.1. NC_009801.1.

    3D structure databases

    ProteinModelPortali A7ZRK3.
    SMRi A7ZRK3. Positions 1-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331111.EcE24377A_3435.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV18902 ; ABV18902 ; EcE24377A_3435 .
    GeneIDi 5590624.
    KEGGi ecw:EcE24377A_3435.
    PATRICi 18296186. VBIEscCol31211_3708.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi PANTLKM.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci ECOL331111:GH7P-3416-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
      Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
      J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E24377A / ETECImported.

    Entry informationi

    Entry nameiA7ZRK3_ECO24
    AccessioniPrimary (citable) accession number: A7ZRK3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3