Reviewed, UniProtKB/Swiss-Prot A7ZR14 (GCST_ECO24)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	EcE24377A_3232

Organism

Escherichia coli O139:H28 (strain E24377A / ETEC) [Complete proteome] [HAMAP]

Taxonomic identifier

331111 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Aminomethyltransferase HAMAP MF_00259		PRO_1000059084

Sequences

	Sequence		Length	Mass (Da)
	A7ZR14-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 1F58C5A244F82242	FASTA	364	40,147
	10 20 30 40 50 60 MAQQTPLYEQ HTLCGARMVD FHGWMMPLHY GSQIDEHHAV RTDAGMFDVS HMTIVDLRGS 70 80 90 100 110 120 RTREFLRYLL ANDVAKLTKS GKALYSGMLN ASGGVIDDLI VYYFTEDFFR LVVNSATREK 130 140 150 160 170 180 DLSWITQHAE PFGIEITVRD DLSMIAVQGP NAQAKAATLF NDAQRQAVEG MKPFFGVQAG 190 200 210 220 230 240 DLFIATTGYT GEAGYEIALP NEKAADFWRA LVEAGVKPCG LGARDTLRLE AGMNLYGQEM 250 260 270 280 290 300 DETISPLAAN MGWTIAWEPA DRDFIGREAL EVQREHGTEK LVGLVMTEKG VLRNELPVRF 310 320 330 340 350 360 TDAQGNQHEG IITSGTFSPT LGYSIALARV PEGIGETAIV QIRNREMPVK VTKPVFVRNG KAVA

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000800 Genomic DNA. Translation: ABV17828.1.
RefSeq	YP_001464243.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7ZR14.
Genome annotation databases
GeneID	5586188.
GenomeReviews	Gene locus EcE24377A_3232 in contig CP000800_GR.
KEGG	ecw:EcE24377A_3232.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VEIRGKW.
Family and domain databases
HAMAP	MF_00259. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_ECO24

Accession

Primary (citable) accession number: A7ZR14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)