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Protein

Ribosomal RNA large subunit methyltransferase M

Gene

rlmM

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei188 – 1881S-adenosyl-L-methionineUniRule annotation
Binding sitei240 – 2401S-adenosyl-L-methionineUniRule annotation
Binding sitei260 – 2601S-adenosyl-L-methionineUniRule annotation
Binding sitei277 – 2771S-adenosyl-L-methionineUniRule annotation
Active sitei306 – 3061Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL331111:GH7P-3095-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase MUniRule annotation (EC:2.1.1.186UniRule annotation)
Alternative name(s):
23S rRNA (cytidine2498-2'-O)-methyltransferaseUniRule annotation
23S rRNA 2'-O-ribose methyltransferase RlmMUniRule annotation
Gene namesi
Name:rlmMUniRule annotation
Ordered Locus Names:EcE24377A_3112
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001122 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Ribosomal RNA large subunit methyltransferase MPRO_1000073561Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7ZQQ1.
SMRiA7ZQQ1. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2244S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. RlmM subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiEOG651SSR.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZQQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVVLLCRP GFEKECAAEI TDKAGQREIF GFARVKENAG YVIYECYQPD
60 70 80 90 100
DGDKLIRELP FSSLIFARQW FVVGELLQHL PPEDRITPIV GMLQGVVEKG
110 120 130 140 150
GELRVEVADT NESKELLKFC RKFTVPLRAA LRDAGVLANY ETPKRPVVHV
160 170 180 190 200
FFIAPGCCYT GYSYSNNNSP FYMGIPRLKF PADAPSRSTL KLEEAFHVFI
210 220 230 240 250
PADEWDERLA NGMWAVDLGA CPGGWTYQLV KRNMWVYSVD NGPMAQSLMD
260 270 280 290 300
TGQVTWLRED GFKFRPTRSN ISWMVCDMVE KPAKVAALMA QWLVNGWCRE
310 320 330 340 350
TIFNLKLPMK KRYEEVSHNL AYIQAQLDEH GINAQIQARQ LYHDREEVTV
360
HVRRIWAAVG GRRDER
Length:366
Mass (Da):41,905
Last modified:October 23, 2007 - v1
Checksum:i595EE105DFB03677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV18799.1.
RefSeqiWP_001045520.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV18799; ABV18799; EcE24377A_3112.
KEGGiecw:EcE24377A_3112.
PATRICi18295540. VBIEscCol31211_3391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV18799.1.
RefSeqiWP_001045520.1. NC_009801.1.

3D structure databases

ProteinModelPortaliA7ZQQ1.
SMRiA7ZQQ1. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV18799; ABV18799; EcE24377A_3112.
KEGGiecw:EcE24377A_3112.
PATRICi18295540. VBIEscCol31211_3391.

Phylogenomic databases

HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiEOG651SSR.

Enzyme and pathway databases

BioCyciECOL331111:GH7P-3095-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiRLMM_ECO24
AccessioniPrimary (citable) accession number: A7ZQQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 23, 2007
Last modified: November 11, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.