ID   HCAF_ECO24              Reviewed;         172 AA.
AC   A7ZPY2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-JAN-2012, entry version 31.
DE   RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit beta;
DE            EC=1.14.12.19;
DE   AltName: Full=Digoxigenin subunit beta;
GN   Name=hcaF; OrderedLocusNames=EcE24377A_2824;
OS   Escherichia coli O139:H28 (strain E24377A / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331111;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E24377A / ETEC;
RX   PubMed=18676672; DOI=10.1128/JB.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic
RT   analysis of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
CC   -!- FUNCTION: Part of the multicomponent 3-phenylpropionate
CC       dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic
CC       acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 3-phenylpropanoate + NADH + O(2) = 3-(cis-5,6-
CC       dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD(+).
CC   -!- CATALYTIC ACTIVITY: (2E)-3-phenylprop-2-enoate + NADH + O(2) =
CC       (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD(+).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate
CC       degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (hcaE and hcaF), a
CC       ferredoxin (hcaC) and a ferredoxin reductase (hcaD) (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; CP000800; ABV20129.1; -; Genomic_DNA.
DR   RefSeq; YP_001463861.1; NC_009801.1.
DR   ProteinModelPortal; A7ZPY2; -.
DR   SMR; A7ZPY2; 2-172.
DR   STRING; A7ZPY2; -.
DR   EnsemblBacteria; EBESCT00000020281; EBESCP00000019343; EBESCG00000019335.
DR   GeneID; 5589475; -.
DR   GenomeReviews; CP000800_GR; EcE24377A_2824.
DR   KEGG; ecw:EcE24377A_2824; -.
DR   PATRIC; 18294941; VBIEscCol31211_3103.
DR   eggNOG; COG5517; -.
DR   GeneTree; EBGT00050000011518; -.
DR   HOGENOM; HBG582498; -.
DR   OMA; DIFAGER; -.
DR   ProtClustDB; PRK10069; -.
DR   BioCyc; ECOL331111:ECE24377A_2824-MONOMER; -.
DR   GO; GO:0008695; F:3-phenylpropionate dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_01649; HcaF; 1; -.
DR   InterPro; IPR023712; HcaF.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   KO; K05709; -.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    172       3-phenylpropionate/cinnamic acid
FT                                dioxygenase subunit beta.
FT                                /FTId=PRO_0000333710.
SQ   SEQUENCE   172 AA;  20579 MW;  83833B22A75BE546 CRC64;
     MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ
     PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQISETDIP NVFAVRVNYL
     LYRAQKERDE TFYVGTRFDK VRRLEDDNWR LLERDIVLDQ AVITSHNLSV LF
//