Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyridoxine kinase

Gene

pdxK

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates.UniRule annotation

Catalytic activityi

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate.UniRule annotation

Cofactori

Zn2+UniRule annotation, Mg2+UniRule annotationNote: Zinc or magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi195 – 1962ATPUniRule annotation
Nucleotide bindingi220 – 23213ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glucokinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. pyridoxal kinase activity Source: UniProtKB-EC
  5. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
  2. pyridoxal 5'-phosphate salvage Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciECOL331111:GH7P-2686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine kinaseUniRule annotation (EC:2.7.1.35UniRule annotation)
Alternative name(s):
PN/PL/PM kinaseUniRule annotation
Pyridoxal kinaseUniRule annotation
Pyridoxamine kinaseUniRule annotation
Vitamin B6 kinaseUniRule annotation
Gene namesi
Name:pdxKUniRule annotation
Ordered Locus Names:EcE24377A_2705
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001122: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Pyridoxine kinasePRO_1000069881Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi331111.EcE24377A_2705.

Structurei

3D structure databases

ProteinModelPortaliA7ZPL9.
SMRiA7ZPL9. Positions 2-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyridoxine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2240.
HOGENOMiHOG000258173.
KOiK00868.
OMAiGRKIYTE.
OrthoDBiEOG69PQ1X.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlP_synth_PyrdxlKinase.
IPR023479. Pyridoxal_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZPL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLLFNDK SRALQADIVA VQSQVVYGSV GNSIAVPAIK QNGLNVFAVP
60 70 80 90 100
TVLLSNTPHY DTFYGGAIPD EWFSGYLRAL QERDALRQLR AVTTGYMGTA
110 120 130 140 150
SQIKILAEWL TALRKDHPDL LIMVDPVIGD IDSGIYVKPD LPEAYRQYLL
160 170 180 190 200
PLAQGITPNI FELEILTGKN CRDLDSAIAA AKSLLSDTLK WVVITSASGN
210 220 230 240 250
EENQEMQVVV VSADSVNVIS HSRVKTDLKG TGDLFCAQLI SGLLKGKALN
260 270 280
DAVHRAGLRV LEVMRYTQQH ESDELILPPL AEA
Length:283
Mass (Da):30,860
Last modified:October 23, 2007 - v1
Checksum:i902F8C55E5B7804B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19738.1.
RefSeqiYP_001463748.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV19738; ABV19738; EcE24377A_2705.
GeneIDi5588451.
KEGGiecw:EcE24377A_2705.
PATRICi18294680. VBIEscCol31211_2975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19738.1.
RefSeqiYP_001463748.1. NC_009801.1.

3D structure databases

ProteinModelPortaliA7ZPL9.
SMRiA7ZPL9. Positions 2-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi331111.EcE24377A_2705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV19738; ABV19738; EcE24377A_2705.
GeneIDi5588451.
KEGGiecw:EcE24377A_2705.
PATRICi18294680. VBIEscCol31211_2975.

Phylogenomic databases

eggNOGiCOG2240.
HOGENOMiHOG000258173.
KOiK00868.
OMAiGRKIYTE.
OrthoDBiEOG69PQ1X.

Enzyme and pathway databases

BioCyciECOL331111:GH7P-2686-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlP_synth_PyrdxlKinase.
IPR023479. Pyridoxal_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiPDXK_ECO24
AccessioniPrimary (citable) accession number: A7ZPL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: January 7, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.