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Protein

Pyridoxine/pyridoxal/pyridoxamine kinase

Gene

pdxK

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

B6-vitamer kinase involved in the salvage pathway of pyridoxal 5'-phosphate (PLP). Catalyzes the phosphorylation of pyridoxine (PN), pyridoxal (PL), and pyridoxamine (PM), forming their respective 5'-phosphorylated esters, i.e. PNP, PLP and PMP.UniRule annotation

Catalytic activityi

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate.UniRule annotation
ATP + pyridoxine = ADP + pyridoxine 5'-phosphate.UniRule annotation
ATP + pyridoxamine = ADP + pyridoxamine 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxal kinase PdxY (pdxY), Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateUniRule annotation
Binding sitei59 – 591SubstrateUniRule annotation
Binding sitei125 – 1251ATPUniRule annotation
Metal bindingi136 – 1361MagnesiumUniRule annotation
Binding sitei157 – 1571ATP; via carbonyl oxygenUniRule annotation
Metal bindingi162 – 1621MagnesiumUniRule annotation
Binding sitei162 – 1621ATPUniRule annotation
Binding sitei195 – 1951ATPUniRule annotation
Binding sitei231 – 2311ATPUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi221 – 2244ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciECOL331111:GH7P-2686-MONOMER.
UniPathwayiUPA01068; UER00298.
UPA01068; UER00299.
UPA01068; UER00300.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine/pyridoxal/pyridoxamine kinaseUniRule annotation (EC:2.7.1.35UniRule annotation)
Short name:
PN/PL/PM kinaseUniRule annotation
Alternative name(s):
B6-vitamer kinaseUniRule annotation
Gene namesi
Name:pdxKUniRule annotation
Ordered Locus Names:EcE24377A_2705
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001122 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Pyridoxine/pyridoxal/pyridoxamine kinasePRO_1000069881Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7ZPL9.
SMRiA7ZPL9. Positions 2-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyridoxine kinase family. PdxK subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000258173.
KOiK00868.
OMAiRIRMEMH.
OrthoDBiEOG69PQ1X.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlP_synth_PyrdxlKinase.
IPR023479. Pyridoxal_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZPL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLLFNDK SRALQADIVA VQSQVVYGSV GNSIAVPAIK QNGLNVFAVP
60 70 80 90 100
TVLLSNTPHY DTFYGGAIPD EWFSGYLRAL QERDALRQLR AVTTGYMGTA
110 120 130 140 150
SQIKILAEWL TALRKDHPDL LIMVDPVIGD IDSGIYVKPD LPEAYRQYLL
160 170 180 190 200
PLAQGITPNI FELEILTGKN CRDLDSAIAA AKSLLSDTLK WVVITSASGN
210 220 230 240 250
EENQEMQVVV VSADSVNVIS HSRVKTDLKG TGDLFCAQLI SGLLKGKALN
260 270 280
DAVHRAGLRV LEVMRYTQQH ESDELILPPL AEA
Length:283
Mass (Da):30,860
Last modified:October 23, 2007 - v1
Checksum:i902F8C55E5B7804B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19738.1.
RefSeqiWP_000096660.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV19738; ABV19738; EcE24377A_2705.
KEGGiecw:EcE24377A_2705.
PATRICi18294680. VBIEscCol31211_2975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19738.1.
RefSeqiWP_000096660.1. NC_009801.1.

3D structure databases

ProteinModelPortaliA7ZPL9.
SMRiA7ZPL9. Positions 2-280.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV19738; ABV19738; EcE24377A_2705.
KEGGiecw:EcE24377A_2705.
PATRICi18294680. VBIEscCol31211_2975.

Phylogenomic databases

HOGENOMiHOG000258173.
KOiK00868.
OMAiRIRMEMH.
OrthoDBiEOG69PQ1X.

Enzyme and pathway databases

UniPathwayiUPA01068; UER00298.
UPA01068; UER00299.
UPA01068; UER00300.
BioCyciECOL331111:GH7P-2686-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlP_synth_PyrdxlKinase.
IPR023479. Pyridoxal_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiPDXK_ECO24
AccessioniPrimary (citable) accession number: A7ZPL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: April 13, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.