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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway:itrehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei77 – 771Glucose-6-phosphateBy similarity
Sitei86 – 861Involved in alpha anomer selectivityBy similarity
Binding sitei131 – 1311Glucose-6-phosphateBy similarity
Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
Binding sitei263 – 2631UDP-glucoseBy similarity
Binding sitei268 – 2681UDP-glucoseBy similarity
Binding sitei301 – 3011Glucose-6-phosphateBy similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL331111:GH7P-2113-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:EcE24377A_2128
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001122 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348897Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA7ZN22.
SMRiA7ZN22. Positions 2-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose bindingBy similarity
Regioni366 – 3705UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZN22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED
60 70 80 90 100
QPLKKVKKGN ITWASFNLSE QDLDEYYNQF SNAVLWPAFH YRLDLVQFQR
110 120 130 140 150
PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN
210 220 230 240 250
LTRVTTRSAK SHTAWGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA
260 270 280 290 300
ELKNVQNIFS VERLDYSKGL PERFLAYEAL LEKYPQHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI
410 420 430 440 450
VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD
460 470
LKQIVPRSAE SQQRDKVATF PKLA
Length:474
Mass (Da):53,611
Last modified:October 23, 2007 - v1
Checksum:i63FFB6F938056F95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19268.1.
RefSeqiWP_001295646.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV19268; ABV19268; EcE24377A_2128.
KEGGiecw:EcE24377A_2128.
PATRICi18293505. VBIEscCol31211_2405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV19268.1.
RefSeqiWP_001295646.1. NC_009801.1.

3D structure databases

ProteinModelPortaliA7ZN22.
SMRiA7ZN22. Positions 2-458.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV19268; ABV19268; EcE24377A_2128.
KEGGiecw:EcE24377A_2128.
PATRICi18293505. VBIEscCol31211_2405.

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciECOL331111:GH7P-2113-MONOMER.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiOTSA_ECO24
AccessioniPrimary (citable) accession number: A7ZN22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 23, 2007
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.