Reviewed, UniProtKB/Swiss-Prot A7ZML3 (ASTB_ECO24)

Names and origin

Protein names

Recommended name:
    N-succinylarginine dihydrolase
    EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	EcE24377A_1967

Organism

Escherichia coli O139:H28 (strain E24377A / ETEC) [Complete proteome] [HAMAP]

Taxonomic identifier

331111 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity.
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_1000065723
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	248	1	By similarity
	Active site	365	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	359	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZML3-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 5E8293820910D15D	FASTA	447	49,212
	10 20 30 40 50 60 MNAWEVNFDG LVGLTHHYAG LSFGNEASTC HRFQVSNPRQ AAKQGLLKMK ALADAGFPQA 70 80 90 100 110 120 VIPPHERPFI PVLRQLGFSG SDEQVLEKVA RQAPHWLSSV SSASPMWVAN AATIAPSADT 130 140 150 160 170 180 LDGKVHLTVA NLNNKFHRSL EAHVTESLLK AIFNDKEKFS VHSALPQVAL LGDEGAANHN 190 200 210 220 230 240 RLGGHYGEPG MQLFVYGREE GNDTRPSRYP ARQTREASEA VARLNQVNPQ QVIFAQQNPD 250 260 270 280 290 300 VIDQGVFHND VIAVSNRQVL FCHQQAFARQ SQLLANLRAR VNGFMAIEVP ATQVSVSDAV 310 320 330 340 350 360 STYLFNSQLL SRDDGSMMLV LPQECREHAG VWGYLNELLA ADNPISELKV FDLRESMANG 370 380 390 400 410 420 GGPACLRLRV VLTEEERRAV NPAVMMNDTL FNALNDWVDR YYRDRLTAAD LADPQLLRGG 430 440 REALDILSQL LNLGSVYPFQ REGGGNG

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000800 Genomic DNA. Translation: ABV17269.1.
RefSeq	YP_001463042.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5585946.
GenomeReviews	Gene locus EcE24377A_1967 in contig CP000800_GR.
KEGG	ecw:EcE24377A_1967.
NMPDR	fig|331111.3.peg.4388.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7ZML3. HFAHHPA.
Family and domain databases
HAMAP	MF_01172. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_ECO24

Accession

Primary (citable) accession number: A7ZML3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)