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A7ZJG7

- RUSA_ECO24

UniProt

A7ZJG7 - RUSA_ECO24

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Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi72 – 721MagnesiumBy similarity
Metal bindingi91 – 911MagnesiumBy similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL331111:GH7P-815-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene namesi
Name:rusA
Ordered Locus Names:EcE24377A_0821
OrganismiEscherichia coli O139:H28 (strain E24377A / ETEC)
Taxonomic identifieri331111 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001122: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000324834Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi331111.EcE24377A_0821.

Structurei

3D structure databases

ProteinModelPortaliA7ZJG7.
SMRiA7ZJG7. Positions 1-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164DNA bindingBy similarity
Regioni66 – 738DNA bindingBy similarity

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

eggNOGiCOG4570.
HOGENOMiHOG000119986.
KOiK01160.
OMAiRGIHYIS.
OrthoDBiEOG66HVH9.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamiPF05866. RusA. 1 hit.
[Graphical view]
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZJG7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRCRRD LDNLQKAAFD ALTKAGFWLD DELVVDYRVV
110 120
KMPVTKGGKL ELTITELGDE
Length:120
Mass (Da):13,791
Last modified:October 23, 2007 - v1
Checksum:iD95FF66BB01171CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV20145.1.
RefSeqiWP_001099708.1. NC_009801.1.
YP_001461946.1. NC_009801.1.

Genome annotation databases

EnsemblBacteriaiABV20145; ABV20145; EcE24377A_0821.
GeneIDi5588919.
KEGGiecw:EcE24377A_0821.
PATRICi18290892. VBIEscCol31211_1114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000800 Genomic DNA. Translation: ABV20145.1 .
RefSeqi WP_001099708.1. NC_009801.1.
YP_001461946.1. NC_009801.1.

3D structure databases

ProteinModelPortali A7ZJG7.
SMRi A7ZJG7. Positions 1-118.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331111.EcE24377A_0821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV20145 ; ABV20145 ; EcE24377A_0821 .
GeneIDi 5588919.
KEGGi ecw:EcE24377A_0821.
PATRICi 18290892. VBIEscCol31211_1114.

Phylogenomic databases

eggNOGi COG4570.
HOGENOMi HOG000119986.
KOi K01160.
OMAi RGIHYIS.
OrthoDBi EOG66HVH9.

Enzyme and pathway databases

BioCyci ECOL331111:GH7P-815-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.70. 1 hit.
InterProi IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view ]
Pfami PF05866. RusA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001007. RusA. 1 hit.
SUPFAMi SSF103084. SSF103084. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E24377A / ETEC.

Entry informationi

Entry nameiRUSA_ECO24
AccessioniPrimary (citable) accession number: A7ZJG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 23, 2007
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3