A7ZE39 (SYL_CAMC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	Ccon26_11910
ORF Names:	CCC13826_0074

Organism

Campylobacter concisus (strain 13826) [Complete proteome] [HAMAP]

Taxonomic identifier

360104 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter concisus

Protein attributes

Sequence length	821 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 821	821	Leucine--tRNA ligase HAMAP-Rule MF_00049		PRO_0000334738
Regions
	Motif	44 – 54	11	"HIGH" region HAMAP-Rule MF_00049
	Motif	589 – 593	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	592	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZE39 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: E00E273EF5B2431C	FASTA	821	94,106
	10 20 30 40 50 60 MAEKRKYEPL KIEKKWQEIW DKNEEFEPKD DLSLPKKYIL SMFPYPSGRI HMGHVRNYSI 70 80 90 100 110 120 GDALARSYRK SGYNVLHPIG FDSFGMPAEN AAIKHKIHPK IWTYENIDYM KKELASLGFS 130 140 150 160 170 180 FSKKRILATS DPLYTKWEQS FFIKMFEKGL VYRKNAIVNW CEYDQTVLAN EQVEDGKCWR 190 200 210 220 230 240 CGNDVVQKEL PGYYFNITKY ASELLDDLKL LEGKWPNQVI TMQENWIGRS YGLEFKFSLD 250 260 270 280 290 300 EASKETLGGK FDGFEVFTTR PDTIYGVSYT ALAPEHPIVK ALLESDKFDE NKKAKIKAIL 310 320 330 340 350 360 NQSPRERQAS DKDGEFLGIY VVHPLTNEKI PVWVANFILA DYGSGAIMAV PAHDQRDFEF 370 380 390 400 410 420 ASKFNLPIKP VVKPLEGESE GSKAYSEYGV AINSELINGL SSEDAKSFII EKFEKDGLGK 430 440 450 460 470 480 RITNYKLRDW GISRQRYWGA PIPVVHCKCC GVVPEKEENL PIALPEDVEI TGEGNPLDKH 490 500 510 520 530 540 PTWKFTKCPK CGKDAIRETD TMDTFVESSW YFARFASDEK TWEQKALDEK SVNYWMNVDQ 550 560 570 580 590 600 YIGGIEHAIL HLLYARFFQK VLRDLGYLRD DEPFENLLTQ GMVLKDGKKM SKSKGNVVDP 610 620 630 640 650 660 DDIINRYGAD TARLFILFAA PPQKELEWND SAVEGAFRFL NRLWEKSQTI KKIDKLPEID 670 680 690 700 710 720 HESLNKDEKF ARLKIYEALK KSTEVFGDTF AFNTLIAACM EALNAINAQD NDDVNAEGFF 730 740 750 760 770 780 IILNLLEPIV PHIANELSEE LFGRKNFTKI AVKEEVFVKD SIALAVTVNG KKRAEFEVTA 790 800 810 820 SENESEILKQ AKQNVAKWLE GKEILKEIYI KGKLVNFVIK G

References

[1]

"Genome sequence of Campylobacter concisus 13826 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13826.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000792 Genomic DNA. Translation: EAT97782.1.
RefSeq	YP_001467047.1. NC_009802.1.
3D structure databases
ProteinModelPortal	A7ZE39.
ModBase	Search...
Protein-protein interaction databases
STRING	360104.CCC13826_0074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EAT97782; EAT97782; CCC13826_0074.
GeneID	5596212.
KEGG	cco:CCC13826_0074.
PATRIC	20028609. VBICamCon95352_1234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
OMA	GWPEQVK.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	CCON360104:GHAC-1278-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 2 hits.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF00133. tRNA-synt_1. 1 hit. PF13603. tRNA-synt_1_2. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_CAMC1

Accession

Primary (citable) accession number: A7ZE39

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program