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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Campylobacter concisus (strain 13826)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (waaD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei260 – 2601UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 1954ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCON360104:GHAC-1248-MONOMER.
UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:Ccon26_11780
ORF Names:CCC13826_0577
OrganismiCampylobacter concisus (strain 13826)
Taxonomic identifieri360104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000001121 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Bifunctional protein HldEPRO_0000335554Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi360104.CCC13826_0577.

Structurei

3D structure databases

ProteinModelPortaliA7ZE26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 315315RibokinaseAdd
BLAST
Regioni340 – 472133CytidylyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
KOiK03272.
OMAiWAKGGDY.
OrthoDBiEOG68Q0W4.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.

Sequencei

Sequence statusi: Complete.

A7ZE26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVKILVV GDLMLDHYIW GSCERISPEA PVQVVKINNE TYTLGGAGNV
60 70 80 90 100
VRNLLSLGAN VSVASVLGDD EAGKKIKEKL AELNVKDELI LTEKGRESSI
110 120 130 140 150
KSRIMASHQQ VVRIDKESVV KINLEDELVL KVKENLANFK AVLLSDYGKG
160 170 180 190 200
VLSEKVCQEI INECVKLKIP VLIDPKGSDY SKYKNATLLT PNKKEASEAT
210 220 230 240 250
NLKIKDKAEL EKAIKQLKEE LNLTYSIITI SEEGIALYDD KLHIFAAKAK
260 270 280 290 300
EVFDVTGAGD TVLATLGYML ANGADIKEAI KIANLAAAVV VAKIGSATAS
310 320 330 340 350
FSEIEQLLNS SFGANFEHKL KSIEELEEIL SQKGKKKVVF TNGCFDILHA
360 370 380 390 400
GHVKYLARAR ELGDLLVVGL NSDASVKRLK GEARPINSQD DRACVLSGLG
410 420 430 440 450
FVDYVVIFDE DTPLNLITKI KPDVLVKGAD YKGKEVVGSD IVKEVRLIDF
460 470
VEGKSTTGII KRIKDAKNDD KK
Length:472
Mass (Da):51,592
Last modified:October 23, 2007 - v1
Checksum:iB40363C0286503EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: EAT97395.1.
RefSeqiWP_012139957.1. NC_009802.1.

Genome annotation databases

EnsemblBacteriaiEAT97395; EAT97395; CCC13826_0577.
KEGGicco:CCC13826_0577.
PATRICi20028583. VBICamCon95352_1221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: EAT97395.1.
RefSeqiWP_012139957.1. NC_009802.1.

3D structure databases

ProteinModelPortaliA7ZE26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360104.CCC13826_0577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEAT97395; EAT97395; CCC13826_0577.
KEGGicco:CCC13826_0577.
PATRICi20028583. VBICamCon95352_1221.

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
KOiK03272.
OMAiWAKGGDY.
OrthoDBiEOG68Q0W4.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.
BioCyciCCON360104:GHAC-1248-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Campylobacter concisus 13826 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13826.

Entry informationi

Entry nameiHLDE_CAMC1
AccessioniPrimary (citable) accession number: A7ZE26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 23, 2007
Last modified: July 6, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.