ID   TGT_CAMC1               Reviewed;         376 AA.
AC   A7ZD89;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   16-JUN-2009, entry version 10.
DE   RecName: Full=Queuine tRNA-ribosyltransferase;
DE            EC=2.4.2.29;
DE   AltName: Full=tRNA-guanine transglycosylase;
DE   AltName: Full=Guanine insertion enzyme;
GN   Name=tgt; OrderedLocusNames=Ccon26_08790; ORFNames=CCC13826_0093;
OS   Campylobacter concisus (strain 13826).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=360104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G.,
RA   Mandrell R.E., On S., Nelson K.E.;
RT   "Genome sequence of Campylobacter concisus 13826 isolated from human
RT   feces.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Exchanges the guanine residue with 7-aminomethyl-7-
CC       deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His
CC       and -Tyr). After this exchange, a cyclopentendiol moiety is
CC       attached to the 7-aminomethyl group of 7-deazaguanine, resulting
CC       in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-
CC       dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + queuine = [tRNA]-queuine +
CC       guanine.
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + 7-aminomethyl-7-carbaguanine
CC       = [tRNA]-7-aminomethyl-7-carbaguanine + guanine.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: tRNA modification; queuosine-tRNA biosynthesis.
CC   -!- SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family.
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DR   EMBL; CP000792; EAT97330.1; -; Genomic_DNA.
DR   RefSeq; YP_001466747.1; -.
DR   GeneID; 5596725; -.
DR   GenomeReviews; CP000792_GR; Ccon26_08790.
DR   KEGG; cco:CCC13826_0093; -.
DR   OMA; A7ZD89; ECVRLPA.
DR   GO; GO:0008479; F:queuine tRNA-ribosyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00168; -; 1.
DR   InterPro; IPR004803; QtRNA_ribo_trans.
DR   InterPro; IPR002616; tRNA_ribo_trans.
DR   Gene3D; G3DSA:3.20.20.105; tRNA_ribo_trans; 1.
DR   PANTHER; PTHR11962; tRNA_ribo_trans; 1.
DR   Pfam; PF01702; TGT; 1.
DR   TIGRFAMs; TIGR00430; Q_tRNA_tgt; 1.
DR   TIGRFAMs; TIGR00449; tgt_general; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glycosyltransferase; Metal-binding;
KW   Queuosine biosynthesis; Transferase; tRNA processing; Zinc.
FT   CHAIN         1    376       Queuine tRNA-ribosyltransferase.
FT                                /FTId=PRO_1000016767.
FT   ACT_SITE     90     90       Nucleophile (By similarity).
FT   METAL       308    308       Zinc (By similarity).
FT   METAL       310    310       Zinc (By similarity).
FT   METAL       313    313       Zinc (By similarity).
FT   METAL       339    339       Zinc (By similarity).
FT   BINDING      91     91       Substrate (By similarity).
SQ   SEQUENCE   376 AA;  42535 MW;  15FAE716F34D1F73 CRC64;
     MKFEVIKKDG NARRGILTTA HSVIQTPVFM PVGTVGAVKS LDAFDMSEIL DAKIILANTY
     HMYLRPGSKV VREFGGLHGF SKFDRSFLTD SGGFQAFSLR SNTKNDDGGI KFKSHIDGST
     HYFTPRSVLD TQYELGSDIM MILDDLVALP AEPKRIDLSI KRTIKWAKEA IDYHKFMQSK
     GVGLQQNIFG IVQGGTDYEA RKFCAEALNE MPFDGLAIGG LSVGESNEAM YDTVEAVMPF
     MDELRPRYLM GVGTPEDLVE NVERGVDMFD CVMPTRNARN GTLFTSFGKI NIKSAKFIND
     HAPIDPQCQC YTCKRYSRGY LNHLFKAREL TFFRLASLHN LHYYLNLMKE MREAIEVGEF
     AKFKRNFYAK RSTDEL
//