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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter concisus (strain 13826)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCCON360104:GHAC-657-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Ccon26_06280
ORF Names:CCC13826_0868
OrganismiCampylobacter concisus (strain 13826)
Taxonomic identifieri360104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000001121 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
BLAST
Chaini31 – 926896Periplasmic nitrate reductasePRO_1000073640Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi360104.CCC13826_0868.

Structurei

3D structure databases

ProteinModelPortaliA7ZCK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7ZCK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKSA AASAACASAG IAVPSSLSAA NEAEKGWRWD KAACRFCGTG
60 70 80 90 100
CGIMVATKDG KIVAVKGDPE APVNRGLNCI KGYFNAKIMY GEDRITHPLL
110 120 130 140 150
RVNEKGEFDK KGKFKQVSWK QAFDVMEAQF RKTYDELGPH GVGVLGSGQY
160 170 180 190 200
TIPEGYAAVK LMKGGFRSNS IDPNARHCMA SAVLGFMQVF GIDEPSGCFD
210 220 230 240 250
DIELTDTVIA WGANMAEMHP ILWARVSDRK LSDPDRVKVV NLSTYSTRTS
260 270 280 290 300
NLADIEIIFA PSSDLAIWNY IAREIVYNHP EMIDEEFVKK HCVFTAGPVD
310 320 330 340 350
IGYGLRPDIH HKKYAPSELD TAATEKSKVL SEAEGVTLSY LGLKAGDTLE
360 370 380 390 400
NKNAAKADAH WQITFEEFKK ALAPYTLDFT AKVAKGDPNE DINEFKKKLK
410 420 430 440 450
ALADLYIEKN RKVVSFWTMG FNQHQRGTWV NEQAYMVHFL LGKQALPGSG
460 470 480 490 500
AFSLTGQPSA CGTAREVGTF VHRLPADMVV GNPKHREITE KLWKLPAGTL
510 520 530 540 550
SGTPGSHYVK MMRDLEDGKV KFIWVQVNNP WQNTANANHW IKAAREMDNF
560 570 580 590 600
IVVSDPYPGI SAKVADLILP TAMIYEKWGA YGNAERRTQH WRQQVLPVGE
610 620 630 640 650
AMPDIWQMLE FSKRFKLKDV WGEKKVNDKV TLPSVLEAAK AMGYSEEDTL
660 670 680 690 700
FDVLFANEDA KKFSANDPIM ENYDNTEVFG DSRKVIGSDG KEFKGYGFFI
710 720 730 740 750
HKYLWEEYRK FGVGHGHDLA DFDTYHRVRG LRWPVVDGKE TQWRFNTKFD
760 770 780 790 800
PYAKKAAPND KFAFYGNKNA ALPTGDLKGV KNQEKTPLAN KAKIFFRPYM
810 820 830 840 850
DPCEMPSKDY PFWLCTGRVL EHWHTGTMTM RVPELYRAVP EALCYMHEDD
860 870 880 890 900
AKKLGVLQNE IVWVESRRGK VKARVDLKGR NKPPVGLVYV PFFDENVFIN
910 920
KVCLDATCPI SKETDYKKCA VKIYKA
Length:926
Mass (Da):104,218
Last modified:October 23, 2007 - v1
Checksum:i04D4862157A3E725
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: EAT97977.1.
RefSeqiWP_012001481.1. NC_009802.1.

Genome annotation databases

EnsemblBacteriaiEAT97977; EAT97977; CCC13826_0868.
KEGGicco:CCC13826_0868.
PATRICi20027415. VBICamCon95352_0662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: EAT97977.1.
RefSeqiWP_012001481.1. NC_009802.1.

3D structure databases

ProteinModelPortaliA7ZCK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360104.CCC13826_0868.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEAT97977; EAT97977; CCC13826_0868.
KEGGicco:CCC13826_0868.
PATRICi20027415. VBICamCon95352_0662.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciCCON360104:GHAC-657-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Campylobacter concisus 13826 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13826.

Entry informationi

Entry nameiNAPA_CAMC1
AccessioniPrimary (citable) accession number: A7ZCK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 23, 2007
Last modified: May 27, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.