Reviewed, UniProtKB/Swiss-Prot A7ZCK4 (NAPA_CAMC1)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Ccon26_06280
ORF Names:	CCC13826_0868

Organism

Campylobacter concisus (strain 13826) [Complete proteome] [HAMAP]

Taxonomic identifier

360104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 926	896	Periplasmic nitrate reductase		PRO_1000073640
Sites
	Metal binding	44	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	47	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZCK4-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 04D4862157A3E725	FASTA	926	104,218
	10 20 30 40 50 60 MNRRDFIKSA AASAACASAG IAVPSSLSAA NEAEKGWRWD KAACRFCGTG CGIMVATKDG 70 80 90 100 110 120 KIVAVKGDPE APVNRGLNCI KGYFNAKIMY GEDRITHPLL RVNEKGEFDK KGKFKQVSWK 130 140 150 160 170 180 QAFDVMEAQF RKTYDELGPH GVGVLGSGQY TIPEGYAAVK LMKGGFRSNS IDPNARHCMA 190 200 210 220 230 240 SAVLGFMQVF GIDEPSGCFD DIELTDTVIA WGANMAEMHP ILWARVSDRK LSDPDRVKVV 250 260 270 280 290 300 NLSTYSTRTS NLADIEIIFA PSSDLAIWNY IAREIVYNHP EMIDEEFVKK HCVFTAGPVD 310 320 330 340 350 360 IGYGLRPDIH HKKYAPSELD TAATEKSKVL SEAEGVTLSY LGLKAGDTLE NKNAAKADAH 370 380 390 400 410 420 WQITFEEFKK ALAPYTLDFT AKVAKGDPNE DINEFKKKLK ALADLYIEKN RKVVSFWTMG 430 440 450 460 470 480 FNQHQRGTWV NEQAYMVHFL LGKQALPGSG AFSLTGQPSA CGTAREVGTF VHRLPADMVV 490 500 510 520 530 540 GNPKHREITE KLWKLPAGTL SGTPGSHYVK MMRDLEDGKV KFIWVQVNNP WQNTANANHW 550 560 570 580 590 600 IKAAREMDNF IVVSDPYPGI SAKVADLILP TAMIYEKWGA YGNAERRTQH WRQQVLPVGE 610 620 630 640 650 660 AMPDIWQMLE FSKRFKLKDV WGEKKVNDKV TLPSVLEAAK AMGYSEEDTL FDVLFANEDA 670 680 690 700 710 720 KKFSANDPIM ENYDNTEVFG DSRKVIGSDG KEFKGYGFFI HKYLWEEYRK FGVGHGHDLA 730 740 750 760 770 780 DFDTYHRVRG LRWPVVDGKE TQWRFNTKFD PYAKKAAPND KFAFYGNKNA ALPTGDLKGV 790 800 810 820 830 840 KNQEKTPLAN KAKIFFRPYM DPCEMPSKDY PFWLCTGRVL EHWHTGTMTM RVPELYRAVP 850 860 870 880 890 900 EALCYMHEDD AKKLGVLQNE IVWVESRRGK VKARVDLKGR NKPPVGLVYV PFFDENVFIN 910 920 KVCLDATCPI SKETDYKKCA VKIYKA

References

[1]	"Genome sequence of Campylobacter concisus 13826 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000792 Genomic DNA. Translation: EAT97977.1.
RefSeq	YP_001466512.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5597288.
GenomeReviews	Gene locus Ccon26_06280 in contig CP000792_GR.
KEGG	cco:CCC13826_0868.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_CAMC1

Accession

Primary (citable) accession number: A7ZCK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 23, 2007
Last modified:	November 25, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)