Reviewed, UniProtKB/Swiss-Prot A7ZCD0 (ISPH_CAMC1)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Ccon26_05450
ORF Names:	CCC13826_1566

Organism

Campylobacter concisus (strain 13826) [Complete proteome] [HAMAP]

Taxonomic identifier

360104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021093

Sequences

	Sequence		Length	Mass (Da)
	A7ZCD0-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F918E8D5D11C2D01	FASTA	274	30,888
	10 20 30 40 50 60 MKIELASSYG FCFGVKRAIK IAENAGDAAT IGPLIHNNEE ISRLEKNYNV KTLEGIDELK 70 80 90 100 110 120 DEKKAIIRTH GITKNDLAEL KKTDIKVIDA TCPFVTKPQQ ICEKMSEEGY DVVIFGDMHH 130 140 150 160 170 180 PEVKGVKSYA KGNVYVVLEE SELEGIKFKQ KVALVSQTTR KVEKFMQIAN YLMLHVKELR 190 200 210 220 230 240 VFNTICNATF ENQEAAKNLA KRADVMIIIG GKNSSNTKQL YLISKNFCED SYLIESEEEL 250 260 270 ERSWFDGKNL CGISAGASTP DWIIQKVVDR IKKV

References

[1]	"Genome sequence of Campylobacter concisus 13826 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000792 Genomic DNA. Translation: EAT99035.1.
RefSeq	YP_001466438.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7ZCD0.
Genome annotation databases
GeneID	5596355.
GenomeReviews	Gene locus Ccon26_05450 in contig CP000792_GR.
KEGG	cco:CCC13826_1566.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KNDICYA.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_CAMC1

Accession

Primary (citable) accession number: A7ZCD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)