Reviewed, UniProtKB/Swiss-Prot A7ZAI1 (IOLA_BACA2)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating]
      Short name=MMSA dehydrogenase
      Short name=MMSDH
      Short name=MSDH
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating]
      Short name=MSA dehydrogenase
    EC=1.2.1.18

Gene names

Name:	iolA
Ordered Locus Names:	RBAM_036780

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Methylmalonate semialdehyde dehydrogenase [acylating] HAMAP MF_01670		PRO_0000352317
Regions
	Nucleotide binding	176 – 180	5	NAD By similarity
Sites
	Active site	284	1	Nucleophile By similarity
	Binding site	382	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZAI1-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: CB86A763E9D08C6F	FASTA	487	53,529
	10 20 30 40 50 60 MAEIRKLKNY INGEWVESKT DQYEDVINPA TKEVMCQVPI STREDVEYAV RSASEAFQTW 70 80 90 100 110 120 SKTAVPRRAR ILFNYQQLLQ QNKEELARLI TLENGKNTTE ALGEVGRGIE NVEFAAGAPS 130 140 150 160 170 180 LMMGDSLASI ATDVEAANYR YPIGVVGGIA PFNFPMMVPC WMFPMAISLG NTFILKPSER 190 200 210 220 230 240 TPLLTEKLAE LFEQAGLPKG VFNVVHGAHD VVNGVLEHPD IKAISFVGSK PVGEYVFKKG 250 260 270 280 290 300 SEHLKRVQAL TGAKNHTIVL NDAHLEDTVT NIIGAAFGSA GERCMACAVV TVEEGIADEF 310 320 330 340 350 360 MAKLQEKAAD IKIGNGLDDG VFLGPVIRED NKKRTHSYIE KGIEEGARLL CDGRENATED 370 380 390 400 410 420 GYFVGPTIFD NVTTDMTIWK DEIFAPVLSV IRVKNLKEAV DIANQSEFAN GACLFTSNAN 430 440 450 460 470 480 AIRYFRENID AGMLGINLGV PAPMAFFPFS GWKSSFFGTL HANGKDSVDF YTRKKVVTAR YPSPDFN

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS76007.1.
RefSeq	YP_001423238.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5462146.
GenomeReviews	Gene locus RBAM_036780 in contig CP000560_GR.
KEGG	bay:RBAM_036780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7ZAI1. GRENATE.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA_BACA2

Accession

Primary (citable) accession number: A7ZAI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)