A7ZAH8 (IOLD_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
Short name=THcHDO hydrolase
EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	RBAM_036750

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity. HAMAP MF_01669
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01669 Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352528
Regions
	Region	442 – 522	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	493	1	Magnesium By similarity
	Metal binding	520	1	Magnesium By similarity
	Binding site	66	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7ZAH8 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F2CAF3ADB1B7016F	FASTA	637	70,158
	10 20 30 40 50 60 MGKTIRLTTA QALIQFLNRQ YIHVDGKEEP FVEGIFTIFG HGNVLGIGQA LEQDAGHLKV 70 80 90 100 110 120 YQGKNEQGMA HAAMAYSKQM LRRKIYAVST SVGPGAANLT AAAGTALANH IPVLLLPADT 130 140 150 160 170 180 FATRQPDPVL QQVEQEYSAA VTTNDALKPV SRYWDRITRP EQLMSSLIRA FEVMTDPAKA 190 200 210 220 230 240 GPATICISQD VEGEAFDFDE SFFEKRVHYI DRMQPSEREL KGAAERIKQS SRPVILVGGG 250 260 270 280 290 300 AKYSGAREEL IALSETYGIP LVETQAGKST VEADFANNLG GMGITGTLAA NKAARQADLI 310 320 330 340 350 360 IGVGTRYTDF ATSSKTAFDF DKAKFLNINV SRMQAYKLDA FQVVADAKVT LGRLHGLLDG 370 380 390 400 410 420 YKSAFGTAIK DWKDEWQAER DRLGKVTFTR DAFEPEIKNH FSQDVLNEYA DALGTELPQT 430 440 450 460 470 480 TALLTINDTI PEDSVVISSA GSLPGDLQRL WHSNVPNTYH LEYGYSCMGY EVSGTLGLKL 490 500 510 520 530 540 AHPDKEVYSL VGDGSFLMLH SELITALQYN KKINVLLFDN SGFGCINNLQ MDHGSGSYFC 550 560 570 580 590 600 EFRTEDNQIL NVDYAKVAEG YGAKTYRANT VEELKAALED AKTQDVSTLI EMKVLPKTMT 610 620 630 DGYDSWWHVG VAEVSEQKSV QRAYEAKETK LKSAKQY

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS76004.1.
RefSeq	YP_001423235.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7ZAH8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7ZAH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006833; EBBACP00000006711; EBBACG00000006825.
GeneID	5462143.
GenomeReviews	Gene locus RBAM_036750 in contig CP000560_GR.
KEGG	bay:RBAM_036750.
PATRIC	18752580. VBIBacAmy31356_3736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3962.
GeneTree	EBGT00070000032034.
HOGENOM	HBG294332.
OMA	PVIRMRR.
ProtClustDB	CLSK2300076.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_036750-MONOMER.
Family and domain databases
HAMAP	MF_01669. IolD. [Tree]
InterPro	IPR023757. THcHDO_hydrolase. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K03336.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_BACA2

Accession

Primary (citable) accession number: A7ZAH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program