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Protein

Fumarate hydratase class II

Gene

citG

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (citG)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:citGImported
Synonyms:fumCUniRule annotation
Ordered Locus Names:RBAM_030160Imported
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)Imported
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7Z8M1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 340Lyase_1InterPro annotationAdd BLAST330
Domaini406 – 459FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A7Z8M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRIEKDTM GEVKVPADKF WGAQTQRSKE NFKIGSEKMP QRVVKAFAIL
60 70 80 90 100
KRSTAIANER LGNLESEKAQ AIAAVCDDVL NGKYDDNFPL VVWQTGSGTQ
110 120 130 140 150
SNMNMNEVVA NRATALLKEK NSELKIHPND DVNRSQSSND TFPTAMHVAA
160 170 180 190 200
VLAVYEQLVP ALDQLRATLD EKAKAYQDIV KIGRTHLQDA TPLTLGQEIS
210 220 230 240 250
GWVYMLDRSK EMILESTEKI RALAIGGTAV GTGINAHPQF GEFVAEEITK
260 270 280 290 300
VTGQTFKSSP NKFHALTSHD EITHVHGALK ALAADLMKIA NDVRWLASGP
310 320 330 340 350
RCGIGEIVIP ENEPGSSIMP GKVNPTQSEA LTMIAAQVMG NDATIGFAAS
360 370 380 390 400
QGNFELNVFK PVIIYNFLQT VQLLSDGMNS FHDKCAVGIE PNQETIQENL
410 420 430 440 450
SNSLMLVTAL NPHIGYENAA KIAKLAHKEG LTLKEAALQL NLLTEEQFNE
460
MVRPEDMVHP KA
Length:462
Mass (Da):50,606
Last modified:October 23, 2007 - v1
Checksum:i85F954B70AF3D86C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA. Translation: ABS75347.1.
RefSeqiWP_007410064.1. NC_009725.1.

Genome annotation databases

EnsemblBacteriaiABS75347; ABS75347; RBAM_030160.
KEGGibay:RBAM_030160.

Similar proteinsi

Entry informationi

Entry nameiA7Z8M1_BACVZ
AccessioniPrimary (citable) accession number: A7Z8M1
Entry historyiIntegrated into UniProtKB/TrEMBL: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 22, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported