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A7Z8E8

- LIPA_BACA2

UniProt

A7Z8E8 - LIPA_BACA2

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Protein
Lipoyl synthase
Gene
lipA, RBAM_029430
Organism
Bacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBAMY326423:GCM4-2941-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:RBAM_029430
OrganismiBacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001120: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Lipoyl synthaseUniRule annotation
PRO_1000012184Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326423.RBAM_029430.

Structurei

3D structure databases

ProteinModelPortaliA7Z8E8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A7Z8E8-1 [UniParc]FASTAAdd to Basket

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MAKKDEHLRK PEWLKIKLNT NENYTGLKKL MRENNLHTVC EEAKCPNIHE    50
CWAVRRTATF MILGSVCTRA CRFCAVKTGL PTELDLQEPE RVADSVALMN 100
LKHAVITAVA RDDQKDGGAG VFAETVRAIR RKSPFTTIEV LPSDMGGNYD 150
NLKTLMDTRP DILNHNIETV RRLTPRVRAR ATYDRSLEFL RRAKEMQPDI 200
PTKSSIMIGL GETKEEIIEV MDDLLANNVD IMAIGQYLQP SKKHLKVQKY 250
YHPDEFAELK EIAMQKGFSH CEAGPLVRSS YHADEQVNEA SKKRQAQA 298
Length:298
Mass (Da):33,893
Last modified:October 23, 2007 - v1
Checksum:iE9B1E7E474496738
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000560 Genomic DNA. Translation: ABS75274.1.
RefSeqiYP_001422505.1. NC_009725.1.

Genome annotation databases

EnsemblBacteriaiABS75274; ABS75274; RBAM_029430.
GeneIDi5462327.
KEGGibay:RBAM_029430.
PATRICi18751070. VBIBacAmy31356_2982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000560 Genomic DNA. Translation: ABS75274.1 .
RefSeqi YP_001422505.1. NC_009725.1.

3D structure databases

ProteinModelPortali A7Z8E8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326423.RBAM_029430.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS75274 ; ABS75274 ; RBAM_029430 .
GeneIDi 5462327.
KEGGi bay:RBAM_029430.
PATRICi 18751070. VBIBacAmy31356_2982.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

BioCyci BAMY326423:GCM4-2941-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 23117 / BGSC 10A6 / FZB42.

Entry informationi

Entry nameiLIPA_BACA2
AccessioniPrimary (citable) accession number: A7Z8E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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