A7Z8C3 (GUAC_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	RBAM_029180

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	GMP reductase HAMAP MF_01511		PRO_1000024490
Regions
	Nucleotide binding	204 – 227	24	NADP Potential
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z8C3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: A2FD907375378B9A	FASTA	326	35,852
	10 20 30 40 50 60 MENVFDYEDI QLIPAKCIVD SRSECDTSVT LGGHTFKLPV VPANMQTIID ENIAAWLAEN 70 80 90 100 110 120 GYFYIMHRFE PEKRLAFVQD MKARGLISSI SVGVKENDYE FIRELKAQEL VPDYITIDIA 130 140 150 160 170 180 HGHSNAVISM IQFIKEHVPE SFVIAGNVGT PEAVRELERA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTHGDIAKSI RFGASMVMIG SLFAGHEESP 250 260 270 280 290 300 GQTIEIDGKL YKEYFGSASE FQKGEKKNVE GKKMHVEHKG SLQDTLVEME QDLQSSISYA 310 320 GGNTLEAIRN VDYVIVKNSI FNGDRF

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS75249.1.
RefSeq	YP_001422480.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z8C3.
SMR	A7Z8C3. Positions 1-321.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z8C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000004819; EBBACP00000004697; EBBACG00000004811.
GeneID	5460081.
GenomeReviews	Gene locus RBAM_029180 in contig CP000560_GR.
KEGG	bay:RBAM_029180.
PATRIC	18751018. VBIBacAmy31356_2956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
GeneTree	EBGT00050000000995.
HOGENOM	HBG298985.
OMA	PDYITID.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_029180-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACA2

Accession

Primary (citable) accession number: A7Z8C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program