A7Z8B5 (AMPA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	RBAM_029100

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019881
Sites
	Active site	273	1	Potential
	Active site	347	1	Potential
	Metal binding	261	1	Manganese 2 By similarity
	Metal binding	266	1	Manganese 1 By similarity
	Metal binding	266	1	Manganese 2 By similarity
	Metal binding	284	1	Manganese 2 By similarity
	Metal binding	343	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z8B5 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 9EF7C758B359C808	FASTA	496	53,370
	10 20 30 40 50 60 MFYASDQLRH PETLVIGLFQ KSTLNGFTKE LDDKLDGHLT QLLKDGDVSA KRNRVSKVYP 70 80 90 100 110 120 PAATGMKRIY FIGLGREADY SFEDTKECFA RVFQQVHQDK KQEVSVLLDT FVSGEVPAAD 130 140 150 160 170 180 AAHALSESCL LAVYEVQDYK HKSNEPDQEL KSVCAVTEED LREVQAGLNV GAAYGQGTNS 190 200 210 220 230 240 ARTLVNMPGN MLTATDLASY AAELAAKYDF ECEILEKDEM EELGMGGLLA VNKGSSEPPK 250 260 270 280 290 300 MIVLKYQGKD QWEDVIGLVG KGITFDTGGY SIKPKTGIVG MKSDMGGAAS VLGAMEIIGE 310 320 330 340 350 360 LRPEQNVLAV IPSTDNMISS DAMKPDDVIV SLSGKTIEIL NTDAEGRLVL ADGITYAKQH 370 380 390 400 410 420 GASVLVDVAT LTGGVIVALG NEMTGAMTNH AAFYEQVAES AKESGEPIWQ LPITEKDKKR 430 440 450 460 470 480 VRNSQMADLN NSPGREGHAI MAGAFIGEFA ENTPWVHLDI AGTATANKAT CFGPAGATGV 490 MARTLAVLTE RFTPER

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS75241.1.
RefSeq	YP_001422472.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z8B5.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z8B5.
Protein family/group databases
MEROPS	M17.010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000004888; EBBACP00000004766; EBBACG00000004880.
GeneID	5461235.
GenomeReviews	Gene locus RBAM_029100 in contig CP000560_GR.
KEGG	bay:RBAM_029100.
PATRIC	18751000. VBIBacAmy31356_2947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
GeneTree	EBGT00050000002613.
HOGENOM	HBG742580.
OMA	CTEEAQL.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_029100-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BACA2

Accession

Primary (citable) accession number: A7Z8B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program