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A7Z7V7 (SYL_BACA2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RBAM_027250
OrganismBacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42) [Complete proteome] [HAMAP]
Taxonomic identifier326423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009291

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7Z7V7 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 37CC64858244D4EF

FASTA80491,270
        10         20         30         40         50         60 
MSFQHEKIEK KWQNYWLDHK TFAVSEENDK PKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMRRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT KQNIDNFRRQ IQSLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPD YYKWTQWIFT KLYEKGLAYV DEVPVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMRQW MLKITAYADR LLEDLEDLDW PESIKDMQRN WIGRSEGANV HFAIDGTDDT 

       250        260        270        280        290        300 
FTVFTTRPDT LFGAAYAVLA PEHELVEAIT TPEQKEAVDA YVKEVQAKSD LERTDLAKTK 

       310        320        330        340        350        360 
TGVFTGAYAV NPANGEKLPI WIADYVLASY GTGAVMAVPA HDERDFEFAK VFSLPVKEVV 

       370        380        390        400        410        420 
KGGNTEEEAY TGDGEHVNSG FLNGLKKAEA IEKMIAWLEE TKNGEKKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGTSTAV PAEELPLILP KTDEIKPSGT GESPLANIKE WVEVTDPETG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYF LRFIDPKNPD QLASPEKLDK WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD IGVVPTKEPF QKLYNQGMIL GENNEKMSKS RGNVVNPDEI VASHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWSESGL DGARRFLDRV WRLFIEENGE LTGKVTEGAG ETLERVYHET 

       670        680        690        700        710        720 
VMKVTENFEA LRFNTGISQL MVFINEAYKA NELPREYIEG FVKLLSPIAP HLAEELWEKL 

       730        740        750        760        770        780 
GHEGSIAYEA WPAYDESKLV DDEVEIVVQL NGKVKAKLMV PADADKAALE QLAQADEKVK 

       790        800 
EQLEGKTIRK IIAVPGKLVN IVAN 

« Hide

References

[1]"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42."
Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R.
Nat. Biotechnol. 25:1007-1014(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 23117 / BGSC 10A6 / FZB42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000560 Genomic DNA. Translation: ABS75083.1.
RefSeqYP_001422314.1. NC_009725.1.

3D structure databases

ProteinModelPortalA7Z7V7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326423.RBAM_027250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS75083; ABS75083; RBAM_027250.
GeneID5462049.
KEGGbay:RBAM_027250.
PATRIC18750606. VBIBacAmy31356_2776.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBAMY326423:GCM4-2723-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACA2
AccessionPrimary (citable) accession number: A7Z7V7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries