A7Z7S3 (MURC_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	RBAM_026910

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004309
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z7S3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 4175E027EF7FAF22	FASTA	432	48,384
	10 20 30 40 50 60 MTVYHFVGIK GTGMSPLAQI LHDTGYQVQG SDIEKQIFTQ AALEKRNIPI YPFSADNIKP 70 80 90 100 110 120 GLTVIAGNAF PDSHPEIAKA LAEGIPVIRY HKFLAEYMKK YTSVAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQQAKPTS FLIGDGTGRG NESSEYFVFE ACEYRRHFLS YQPDYAIMTN IDFDHPDYFA 190 200 210 220 230 240 NIDDVFDAFQ NMALQVNKGI IACGDDEYLP KIHANVPVVY YGTGEENDFQ ARNIVKNTEG 250 260 270 280 290 300 TTFDVFVRNT FYDTFYIPAY GHHNVLNSLA VIALCHYEEI DVNMIKLGLE SFGGVKRRFN 310 320 330 340 350 360 EKVIGSQVLI DDYAHHPTEI KVTIEAARQK YPEREIIAVF QPHTFTRTQS FLDEFAESLS 370 380 390 400 410 420 AADRVYLCDI FGSARENVGK LTITDLQEKI ANAELIEEND TSVLKAHEGA VLIFMGAGDI 430 QKYMRAYENV IA

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS75049.1.
RefSeq	YP_001422280.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z7S3.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z7S3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000004877; EBBACP00000004755; EBBACG00000004869.
GeneID	5459602.
GenomeReviews	Gene locus RBAM_026910 in contig CP000560_GR.
KEGG	bay:RBAM_026910.
PATRIC	18750532. VBIBacAmy31356_2739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
GeneTree	EBGT00050000000630.
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_026910-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACA2

Accession

Primary (citable) accession number: A7Z7S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program