Reviewed, UniProtKB/Swiss-Prot A7Z7R1 (SYY_BACA2)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	RBAM_026790

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_1000088576
Regions
	Domain	355 – 421	67	S4 RNA-binding
	Motif	40 – 49	10	"HIGH" region HAMAP MF_02006
	Motif	232 – 236	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	35	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	174	1	Tyrosine By similarity
	Binding site	235	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z7R1-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 86BF61436EA25779	FASTA	421	47,756
	10 20 30 40 50 60 MTNLLEDLSF RGLIQQMTDE EGLNKQLNEE KIRLYSGFDP TADSLHIGHL LPILTLRRFQ 70 80 90 100 110 120 LAGHHPIALV GGATGLIGDP SGKKAERTLN TQDIVVEWSQ KIKNQLSRFL DFEAGENPAV 130 140 150 160 170 180 IANNFDWIGK LSIIEFLRDV GKNFGINYML AKDTVSSRIE TGISYTEFSY MILQSYDFLN 190 200 210 220 230 240 LYREKNCKLQ IGGSDQWGNI TAGLELIRKS EEEGAKAFGL TIPLVTKADG TKFGKTEGGA 250 260 270 280 290 300 IWLDKEKTSP YEFYQFWINT DDRDVVKYLK YFTFLSKEEI EAYAEKTETA PEKREAQKRL 310 320 330 340 350 360 AEEVTVLVHG REALEQAVHI SQALFSGNIK ELSAQDVKVG FKDVPSFEKD RSEELSLVDV 370 380 390 400 410 420 LVESKLSPSK RQAREDIQNG AVYINGERQT DIGHLLTAED RIEDQFTVLR RGKKKYFLIT Y

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS75037.1.
RefSeq	YP_001422268.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z7R1.
Genome annotation databases
GeneID	5460229.
GenomeReviews	Gene locus RBAM_026790 in contig CP000560_GR.
KEGG	bay:RBAM_026790.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TFYIGFD.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BACA2

Accession

Primary (citable) accession number: A7Z7R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)