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Reviewed, UniProtKB/Swiss-Prot A7Z7M0 (ASSY_BACA2)

Last modified February 9, 2010. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase
Gene names
Name: argG
Ordered Locus Names: RBAM_026380
OrganismBacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]
Taxonomic identifier326423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005

Subunit structure

Homotetramer By similarity. HAMAP MF_00005

Subcellular location

Cytoplasm Probable HAMAP MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Argininosuccinate synthase HAMAP MF_00005
PRO_1000000382

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site871Citrulline By similarity
Binding site1171ATP; via amide nitrogen By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site2601Citrulline By similarity
Binding site2721Citrulline By similarity

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Sequences

Sequence LengthMass (Da)Tools
A7Z7M0-1 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 71295799DE62CDC7

FASTA40344,630
        10         20         30         40         50         60 
MAEQKKVVLA YSGGLDTSVA IKWLQEQGYD VVACCLDVGE GKDLAFVQQK ALEVGAVNSY 

        70         80         90        100        110        120 
VIDAKEEFAR EYALISMQAH TMYEGKYPLV SALSRPLIAK KLVEVAEKEN AVAVAHGCTG 

       130        140        150        160        170        180 
KGNDQVRFEV SIKSLNPDLE VIAPVREWQW SREEEIEYAA SRGIPIPINL DSPYSIDQNL 

       190        200        210        220        230        240 
WGRANECGIL EDPWAAPPEG AYDLTASLEN TPDVPEIIEI AFEAGVPVSI DGVTYPLADL 

       250        260        270        280        290        300 
ILKLNETAGK HGIGRIDHVE NRLVGIKSRE VYECPGAMTL ITAHKELEDL TLVKEVAHFK 

       310        320        330        340        350        360 
PAIEQKLSEI IYNGLWFSPL KDALLAFLKE TQKHVTGVVR VKLFKGHAIV EGRKSEYSLY 

       370        380        390        400 
DEKLATYTKD DAFDHHAAIG FIELWGLPTK VNSIVKKKEQ IEA

« Hide

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References

[1]"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42."
Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R.
Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000560 Genomic DNA. Translation: ABS74996.1.
RefSeqYP_001422227.1.

3D structure databases

SMRA7Z7M0. Positions 6-397.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7Z7M0.

Genome annotation databases

GeneID5461722.
GenomeReviewsGene locus RBAM_026380 in contig CP000560_GR.
KEGGbay:RBAM_026380.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHBG335267.
OMAIAPVREW.

Family and domain databases

HAMAPMF_00005. Arg_succ_synth_type1.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameASSY_BACA2
AccessionPrimary (citable) accession number: A7Z7M0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents