Reviewed, UniProtKB/Swiss-Prot A7Z7I1 (SYT_BACA2)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	RBAM_025990

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020341
Regions
	Region	245 – 542	298	Catalytic HAMAP MF_00184
Sites
	Metal binding	338	1	Zinc; catalytic By similarity
	Metal binding	389	1	Zinc; catalytic By similarity
	Metal binding	519	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z7I1-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: A2E34C4A029D7E11	FASTA	643	73,744
	10 20 30 40 50 60 MSDMINITFP DGAVKEFPKG TTTEDIAASI SPGLKKKSLA GKLNGQEIDL RTPIQEDGAV 70 80 90 100 110 120 EIITEGTEEG LNIMRHSTAH LLAQAIKRIY KDVKFGVGPV IENGFYYDVE MEQALTPDDL 130 140 150 160 170 180 PKIEKEMKRI VGENLPIIRK EVSRDEAKAM FAEIGDNLKL ELLDAIPEDE TVSIYEQGEF 190 200 210 220 230 240 FDLCRGVHVP STGKIKEFKL LSLAGAYWRG DSKNQMLQRI YGTAFFKKDD LKEHLRMLEE 250 260 270 280 290 300 AKERDHRKLG KELKLFANSQ KVGQGLPLWL PKGATIRRVI ERYIVDKELS LGYEHVYTPV 310 320 330 340 350 360 LGSKDLYETS GHWDHYQDTM FPPMEMDNET LVLRPMNCPH HMMIYKQDIH SYRELPIRIA 370 380 390 400 410 420 ELGTMHRYEM SGALSGLQRV RGMTLNDAHI FVRPDQIKEE FIRTVRLIQD VYEDFGLNDY 430 440 450 460 470 480 TFRLSYRDPE DTEKYFDDDE MWNKAQSMLK AAMDEMGHDY YEAEGEAAFY GPKLDVQVKT 490 500 510 520 530 540 AIGKEETLST VQLDFLLPER FDLTYVGEDG KQHRPVVIHR GVVSTMERFV AFLIEEHKGA 550 560 570 580 590 600 LPTWLAPVQF QVIPVSPSVH LDYAKKVQER LQREGLRVEL DSRDEKIGYK IREAQMQKIP 610 620 630 640 YMLVVGDQEA ENGAVNVRKY GEQKSETVSL DDFVKKAVAE AKK

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74957.1.
RefSeq	YP_001422188.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z7I1.
Genome annotation databases
GeneID	5460671.
GenomeReviews	Gene locus RBAM_025990 in contig CP000560_GR.
KEGG	bay:RBAM_025990.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IQLDFML.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BACA2

Accession

Primary (citable) accession number: A7Z7I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)