A7Z7C6 (RNPH_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	RBAM_025440

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease PH HAMAP MF_00564		PRO_1000024776

Sequences

	Sequence		Length	Mass (Da)
	A7Z7C6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 25BD2E8B1C65FFF6	FASTA	245	26,717
	10 20 30 40 50 60 MRHDGRQHDE LRPITFDLDF ITHPEGSVLI TAGNTKVICN ASVEDRVPPF LRGGGKGWIT 70 80 90 100 110 120 AEYSMLPRAT NQRTIRESSK GKVSGRTMEI QRLIGRALRA VVDLEKLGER TIWIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAFLAMAIA IGKLVKSGVI KTSPVTDYLA AISVGMDKEE GLLLDLNYEE 190 200 210 220 230 240 DSAAEVDMNI IMTGSGRFVE LQGTGEEATF SREDLNGLLS LAEKGIQTLI QKQKEVLGET LPELK

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74902.1.
RefSeq	YP_001422133.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z7C6.
SMR	A7Z7C6. Positions 1-242.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z7C6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000004334; EBBACP00000004212; EBBACG00000004326.
GeneID	5461065.
GenomeReviews	Gene locus RBAM_025440 in contig CP000560_GR.
KEGG	bay:RBAM_025440.
PATRIC	18750210. VBIBacAmy31356_2578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000001825.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_025440-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BACA2

Accession

Primary (citable) accession number: A7Z7C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program