A7Z7B9 (ILVC_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	RBAM_025350

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050479
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z7B9 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 360EE4AFB8ECE588	FASTA	342	37,473
	10 20 30 40 50 60 MVKVYYNGDI KENVLAGKKV AIIGYGSQGH AHALNLKESG IDVIVGVRQG KSFTQAQEDG 70 80 90 100 110 120 HQVFSVREAA AQADIIMVLL PDEQQQKVYE AEIKDELTAG KSLVFAHGFN VHFHQIVPPA 130 140 150 160 170 180 DVDVFLVAPK GPGHLVRRTY EQGAGVPALF AIYQDVSGEA KDTALAYAKG IGGARAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GLTALVKAGF ETLTEAGYQP ELAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 EGLAGMRYSI SDTAQWGDFV SGPRVVDAKV KESMKQVLTD IQNGTFAKEW IVENQVNRPR 310 320 330 340 FNAINASENE HQIEKVGRQL REMMPFVKQG KKKEAVVSVA QN

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74895.1.
RefSeq	YP_001422126.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z7B9.
SMR	A7Z7B9. Positions 2-327.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z7B9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006771; EBBACP00000006649; EBBACG00000006763.
GeneID	5462136.
GenomeReviews	Gene locus RBAM_025350 in contig CP000560_GR.
KEGG	bay:RBAM_025350.
PATRIC	18750190. VBIBacAmy31356_2570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
GeneTree	EBGT00050000000943.
HOGENOM	HBG286525.
OMA	EYANGRG.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_025350-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACA2

Accession

Primary (citable) accession number: A7Z7B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program