Reviewed, UniProtKB/Swiss-Prot A7Z7B9 (ILVC_BACA2)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	RBAM_025350

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050479
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z7B9-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 360EE4AFB8ECE588	FASTA	342	37,473
	10 20 30 40 50 60 MVKVYYNGDI KENVLAGKKV AIIGYGSQGH AHALNLKESG IDVIVGVRQG KSFTQAQEDG 70 80 90 100 110 120 HQVFSVREAA AQADIIMVLL PDEQQQKVYE AEIKDELTAG KSLVFAHGFN VHFHQIVPPA 130 140 150 160 170 180 DVDVFLVAPK GPGHLVRRTY EQGAGVPALF AIYQDVSGEA KDTALAYAKG IGGARAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GLTALVKAGF ETLTEAGYQP ELAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 EGLAGMRYSI SDTAQWGDFV SGPRVVDAKV KESMKQVLTD IQNGTFAKEW IVENQVNRPR 310 320 330 340 FNAINASENE HQIEKVGRQL REMMPFVKQG KKKEAVVSVA QN

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74895.1.
RefSeq	YP_001422126.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5462136.
GenomeReviews	Gene locus RBAM_025350 in contig CP000560_GR.
KEGG	bay:RBAM_025350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7Z7B9. KDELTAG.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACA2

Accession

Primary (citable) accession number: A7Z7B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)