Reviewed, UniProtKB/Swiss-Prot A7Z766 (TGT_BACA2)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	RBAM_024820

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016763
Sites
	Active site	96	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	339	1	Zinc By similarity
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z766-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 01E35AD4B9C5DFAB	FASTA	381	43,666
	10 20 30 40 50 60 MAEQPIRYEF IKECKQTGAR LGRVHTPHGS FETPVFMPVG TLATVKTMSP DELKSMDAGI 70 80 90 100 110 120 ILSNTYHLWL RPGHDIVKEA GGLHKFMNWD RAILTDSGGF QVFSLSKFRN IEEEGVHFRN 130 140 150 160 170 180 HLNGDKLFLS PEKAMEIQNA LGSDIMMAFD ECPPYPAEYD YMKRSVERTS RWAERCLEAH 190 200 210 220 230 240 GRSDEQGLFG IVQGGEYEDL RTQSAKDLVS LDFPGYAIGG LSVGEPKHVM NRVLEFTTPL 250 260 270 280 290 300 LPKDKPRYLM GVGSPDALID GAIRGVDMFD CVLPTRIARN GTVFTNEGRL NMKNAKYERD 310 320 330 340 350 360 FRPIDEECSC HTCKNYSRAY IRHLIRCNET FGIRLTTYHN LHFLLHLMEQ VRQAIREDRL 370 380 GDFREEFFER YGYNKPNAKS F

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74842.1.
RefSeq	YP_001422073.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5462891.
GenomeReviews	Gene locus RBAM_024820 in contig CP000560_GR.
KEGG	bay:RBAM_024820.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7Z766. MAFDQCP.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BACA2

Accession

Primary (citable) accession number: A7Z766

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)