Reviewed, UniProtKB/Swiss-Prot A7Z750 (SYD_BACA2)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	RBAM_024660

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000006635

Sequences

	Sequence		Length	Mass (Da)
	A7Z750-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 6AA5F595964374AD	FASTA	592	66,058
	10 20 30 40 50 60 MFGRTYYCGE ITEKAIGETV KLKGWVQKRR DLGGLIFIDL RDRSGIVQVV FNPDVSKEAL 70 80 90 100 110 120 AAAEAIRNEY VLDIEGTVSA REEGTVNPNL KTGKIEIKAE AVTVLSTAKT PPFPISDQAA 130 140 150 160 170 180 DVSEDVRLKH RYLDLRRPAM FQTLQMRHQV TKSIRHFLDE NGFLDIETPI LTKSSPEGAR 190 200 210 220 230 240 DYLVPSRVHE GEFYALPQSP QIFKQLLMVS GFERYYQVAR CFRDEDLRAD RQPEFTQIDI 250 260 270 280 290 300 EMSFMSQEDI MALAEEMMAK VMLETKGVEV PLPLPRMTYE EAMNSYGSDK PDTRFGMLLT 310 320 330 340 350 360 DVSDIVKDTE FKVFSSAVAN GGVVKAINVK GAAGDYSRKD IDALGVFAGN YGAKGLAWVK 370 380 390 400 410 420 VEADGVKGPI AKFFDEEKQA KLAEALEASE GDLLLFGADK FEVVAQSLGA LRLKLGKERG 430 440 450 460 470 480 LIDESLFNFL WVVDWPLLEH DEEEGRFYAA HHPFTMPVRE DLDLIETAPE DMKAQAYDLV 490 500 510 520 530 540 LNGYELGGGS IRIFEKDVQE KMFGLLGFTK EEAEEEFGYL LEAFEYGAPP HGGIALGLDR 550 560 570 580 590 LVMLLAGRTN LRDTIAFPKT ASASCLLTEA PGEVSDAQLE ELHLSVKRKV KQ

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74826.1.
RefSeq	YP_001422057.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z750.
Genome annotation databases
GeneID	5462061.
GenomeReviews	Gene locus RBAM_024660 in contig CP000560_GR.
KEGG	bay:RBAM_024660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACA2

Accession

Primary (citable) accession number: A7Z750

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)