A7Z735 (SYA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	RBAM_024510

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347497
Sites
	Metal binding	566	1	Zinc Potential
	Metal binding	570	1	Zinc Potential
	Metal binding	668	1	Zinc Potential
	Metal binding	672	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z735 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 07F9E7B93214AB3D	FASTA	878	96,918
	10 20 30 40 50 60 MKHLTSAEVR QMFLDFFKEK GHAVEPSASL VPHEDPSLLW INSGVATLKK YFDGRVVPEN 70 80 90 100 110 120 PRIVNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAITWAWE FLTSEKWIGF 130 140 150 160 170 180 DPELLSVTVH PEDEEAYTLW AEKIGVPEER IIRLEGNFWD IGEGPSGPNT EIFYDRGEKY 190 200 210 220 230 240 GNDPSDSELY PGGENDRYLE VWNLVFSEFN HNPDGTYTPL PKKNIDTGMG LERMVSVIQD 250 260 270 280 290 300 VSTNFDTDLF MPIIEATERI SGDAYGKDAV KDTAFKVIAD HIRTVAFAVS DGALPSNEGR 310 320 330 340 350 360 GYVLRRLLRR AVRYAKTLGI SRPFMYELVP TVAGIMDAFY PEVKEKQEFI AKVIKTEEER 370 380 390 400 410 420 FHETLNEGLA ILSDVIKKEK EKGSGIISGK DVFKLYDTYG FPVELTEEYA EDEQMTVDHK 430 440 450 460 470 480 GFEAEMEKQR ERARNARQDV GSMQVQGGAL RDITAESTFV GYSAVKAEAK VIELLHDGQL 490 500 510 520 530 540 ISEAHEGDTV QILLDKTPFY AESGGQIGDR GVLRSEQAVV TIKDVKKAPN GQHVHEGTVD 550 560 570 580 590 600 SGTIQKGASV TAEVEDQMRS GVVKNHTATH LLHQALKDVL GTHVNQAGSL VTENRLRFDF 610 620 630 640 650 660 SHFGQVTKEE LERIEGIVNE KIWESIPVAI DLKPINEAKE MGAMALFGEK YGDIVRVVQV 670 680 690 700 710 720 GDYSLELCGG CHVTNTAEIG LFKIVSESGI GAGTRRIEAV TGKGAYQEMN SQLSLLQHAA 730 740 750 760 770 780 DELKSNVKDV PKRIQSLQAE LKEAQRENES LLSKLGNVEA GAILSKVKDI GGVKVLAEKV 790 800 810 820 830 840 NAKDMNHLRT MVDDLKAKLG SAVIILGAVQ NEKVNLSAGV TKDLIEKGLH AGKMVKQAAE 850 860 870 VCGGGGGGRP DMAQAGGKHP EKLEEALASA VDWIKSVL

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74811.1.
RefSeq	YP_001422042.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z735.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z735.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006772; EBBACP00000006650; EBBACG00000006764.
GeneID	5461310.
GenomeReviews	Gene locus RBAM_024510 in contig CP000560_GR.
KEGG	bay:RBAM_024510.
PATRIC	18750012. VBIBacAmy31356_2481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
GeneTree	EBGT00050000001776.
HOGENOM	HBG354397.
OMA	MFTNSGM.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_024510-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACA2

Accession

Primary (citable) accession number: A7Z735

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program