A7Z6U1 (SYGA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	RBAM_023570

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000047397

Sequences

	Sequence		Length	Mass (Da)
	A7Z6U1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 7A1F11BF5FBFE356	FASTA	295	34,147
	10 20 30 40 50 60 MNIQDMILTL QKHWSNEGCV LMQSYDVEKG AGTMSPYTFL RSIGPEPWRV AYVEPSRRPA 70 80 90 100 110 120 DGRYGENPNR LYQHHQFQVI IKPSPDNIQE LYLDSLRALG IDPLEHDIRF VEDNWENPSL 130 140 150 160 170 180 GCAGLGWEVW LDGMEITQFT YFQQVGGIEC KPVSVEITYG IERLASYIQD KENVFDLEWT 190 200 210 220 230 240 SGFTVKDLFM MAEYEHSVYT FETSDVDMLF HLFSTYEKEA IRQMDNGLVH PAYDYVLKCS 250 260 270 280 290 HTFNLLDAKG AISVTERTGY IGRVRNLARK VAKTYYEERE KLGFPMLKEE GSSHE

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74717.1.
RefSeq	YP_001421948.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z6U1.
SMR	A7Z6U1. Positions 1-281.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z6U1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000005155; EBBACP00000005033; EBBACG00000005147.
GeneID	5461365.
GenomeReviews	Gene locus RBAM_023570 in contig CP000560_GR.
KEGG	bay:RBAM_023570.
PATRIC	18749810. VBIBacAmy31356_2380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
GeneTree	EBGT00050000002437.
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_023570-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_BACA2

Accession

Primary (citable) accession number: A7Z6U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program