Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A7Z6S8

- END4_BACA2

UniProt

A7Z6S8 - END4_BACA2

Protein

Probable endonuclease 4

Gene

nfo

Organism
Bacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

    Cofactori

    Binds 3 zinc ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691Zinc 1UniRule annotation
    Metal bindingi110 – 1101Zinc 1UniRule annotation
    Metal bindingi145 – 1451Zinc 1UniRule annotation
    Metal bindingi145 – 1451Zinc 2UniRule annotation
    Metal bindingi179 – 1791Zinc 2UniRule annotation
    Metal bindingi182 – 1821Zinc 3UniRule annotation
    Metal bindingi214 – 2141Zinc 2UniRule annotation
    Metal bindingi227 – 2271Zinc 3UniRule annotation
    Metal bindingi229 – 2291Zinc 3UniRule annotation
    Metal bindingi259 – 2591Zinc 2UniRule annotation

    GO - Molecular functioni

    1. deoxyribonuclease IV (phage-T4-induced) activity Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAMY326423:GCM4-2342-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
    Alternative name(s):
    Endodeoxyribonuclease IVUniRule annotation
    Endonuclease IVUniRule annotation
    Gene namesi
    Name:nfoUniRule annotation
    Ordered Locus Names:RBAM_023440
    OrganismiBacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
    Taxonomic identifieri326423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001120: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Probable endonuclease 4PRO_1000011288Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi326423.RBAM_023440.

    Structurei

    3D structure databases

    ProteinModelPortaliA7Z6S8.
    SMRiA7Z6S8. Positions 2-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AP endonuclease 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0648.
    HOGENOMiHOG000224895.
    KOiK01151.
    OMAiIAWLKSE.
    OrthoDBiEOG6Z0QCM.

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    HAMAPiMF_00152. Nfo.
    InterProiIPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view]
    PANTHERiPTHR21445. PTHR21445. 1 hit.
    PfamiPF01261. AP_endonuc_2. 1 hit.
    [Graphical view]
    SMARTiSM00518. AP2Ec. 1 hit.
    [Graphical view]
    SUPFAMiSSF51658. SSF51658. 1 hit.
    TIGRFAMsiTIGR00587. nfo. 1 hit.
    PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7Z6S8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKIGSHVSM SGKHMLLAAS KEAVSYGANT FMIYTGAPQN TRRKKIEDLN    50
    IEAGRAHMKE NGIDEIVVHA PYIINIGNTT NPATFELGVD FLRSEIERTA 100
    AIGARQIVLH PGAHVGAGAE TGIKKIIEGL NEVIVPGQNV QIALETMAGK 150
    GSECGRSFEE LAEIIAGVTH NEHLSVCFDT CHTHDAGYDV ANDFDGVLNE 200
    FDKIVGIDRI KVLHVNDSKN IKGARKDRHE NIGFGEIGFD ALQYIVHHEQ 250
    LADIPKILET PYVGEDKKNK KPPYRFEIEM LKEKQFDKEL LEKISAQ 297
    Length:297
    Mass (Da):32,792
    Last modified:October 23, 2007 - v1
    Checksum:iC8A3FC59BE4E4DEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000560 Genomic DNA. Translation: ABS74704.1.
    RefSeqiYP_001421935.1. NC_009725.1.

    Genome annotation databases

    EnsemblBacteriaiABS74704; ABS74704; RBAM_023440.
    GeneIDi5461457.
    KEGGibay:RBAM_023440.
    PATRICi18749784. VBIBacAmy31356_2367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000560 Genomic DNA. Translation: ABS74704.1 .
    RefSeqi YP_001421935.1. NC_009725.1.

    3D structure databases

    ProteinModelPortali A7Z6S8.
    SMRi A7Z6S8. Positions 2-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 326423.RBAM_023440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS74704 ; ABS74704 ; RBAM_023440 .
    GeneIDi 5461457.
    KEGGi bay:RBAM_023440.
    PATRICi 18749784. VBIBacAmy31356_2367.

    Phylogenomic databases

    eggNOGi COG0648.
    HOGENOMi HOG000224895.
    KOi K01151.
    OMAi IAWLKSE.
    OrthoDBi EOG6Z0QCM.

    Enzyme and pathway databases

    BioCyci BAMY326423:GCM4-2342-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    HAMAPi MF_00152. Nfo.
    InterProi IPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view ]
    PANTHERi PTHR21445. PTHR21445. 1 hit.
    Pfami PF01261. AP_endonuc_2. 1 hit.
    [Graphical view ]
    SMARTi SM00518. AP2Ec. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51658. SSF51658. 1 hit.
    TIGRFAMsi TIGR00587. nfo. 1 hit.
    PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 23117 / BGSC 10A6 / FZB42.

    Entry informationi

    Entry nameiEND4_BACA2
    AccessioniPrimary (citable) accession number: A7Z6S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3