A7Z6J5 (DXS_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	RBAM_022600

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019008

Sequences

	Sequence		Length	Mass (Da)
	A7Z6J5 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 05E66B3E431664C1	FASTA	633	69,717
	10 20 30 40 50 60 MDLLSIQDPS FLKKMSIEQL EELSEEIRNF LITSLSASGG HIGPNLGVVE LTIALHKEFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKLL TGRGKEFETL RQYKGLCGFP KRSESEHDVW ETGHSSTSLS 130 140 150 160 170 180 GAMGMAAARD IKGSKEYIIP IIGDGALTGG MALEALNHIG DEKKDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGAIHSMLGR LRTAGKYQWV KDELEYLFKR IPAVGGKLAA TAERIKDSLK YMLVSGMFFE 250 260 270 280 290 300 ELGFTYLGPV DGHSYHELFE NLQYAKKTKG PVLLHVITKK GKGYKPAETD TIGTWHGTGP 310 320 330 340 350 360 YKINTGDFVK PKAAAPSWSG LVSGTVQELA REDDRIVAIT PAMPVGSKLE GFAKEFPERM 370 380 390 400 410 420 FDVGIAEQHA ATMAAGMALQ GMKPFLAIYS TFLQRAYDQV VHDICRQNAN VFIGIDRAGL 430 440 450 460 470 480 VGADGETHQG VFDIAFLRHI PNLVLMMPKD ENEGRHMVNT ALNYEEGPIA MRFPRGNGLG 490 500 510 520 530 540 VKMDKELKTI PIGTWEVLRP GKDAVILTFG TTIEMALEAA EELQKEGLSV RVVNARFIKP 550 560 570 580 590 600 IDKQMMKAIL NEGLPILTIE EAVLEGGFGS TILEYAHDLG MYHTPIDRMG IPDRFIEHGS 610 620 630 VTALLEEIGL TKAEVMNRIK LLMPPKTHKG IGS

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74621.1.
RefSeq	YP_001421852.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z6J5.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z6J5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007731; EBBACP00000007609; EBBACG00000007723.
GeneID	5462341.
GenomeReviews	Gene locus RBAM_022600 in contig CP000560_GR.
KEGG	bay:RBAM_022600.
PATRIC	18749610. VBIBacAmy31356_2281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
OMA	RAHEFES.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_022600-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACA2

Accession

Primary (citable) accession number: A7Z6J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program