A7Z6E7 (NAMA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Synonyms:	yqjM
Ordered Locus Names:	RBAM_022120

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	NADPH dehydrogenase HAMAP MF_01614		PRO_0000323519
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z6E7 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 662EF82ED4CAAAC2	FASTA	338	37,643
	10 20 30 40 50 60 MARKLFTPWT VKDVTIKNRI VMAPMCMYSS HEKDGKLQPF HMAHYISRAI GQVGLIIVEA 70 80 90 100 110 120 TAVNPQGRIS DQDLGIWSDD HIEGFAKLTE QVKAQGSKIG IQLAHAGRKA ELEGDIYAPS 130 140 150 160 170 180 AIPFDEQSKT PAEMTTEQIK ETIQEFKQAA ARAKEAGFDI IELHAAHGYL MHEFLSPLSN 190 200 210 220 230 240 HRTDEYGGSH ENRYRFLGET IEAVKEVWDG PLFVRISASD YTDKGLDIAD HIGFAKWMKE 250 260 270 280 290 300 QGVDLIDCSS GALVQADINV FPGYQVSFAE KIREQAEIAT GAVGLITTGT MAEEILQNNR 310 320 330 ADLIFVAREL LRDPHFARSA AKQLNTEIPS PVQYDRAW

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74573.1.
RefSeq	YP_001421804.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z6E7.
SMR	A7Z6E7. Positions 2-338.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z6E7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007830; EBBACP00000007708; EBBACG00000007822.
GeneID	5459994.
GenomeReviews	Gene locus RBAM_022120 in contig CP000560_GR.
KEGG	bay:RBAM_022120.
PATRIC	18749510. VBIBacAmy31356_2231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1902.
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_022120-MONOMER.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACA2

Accession

Primary (citable) accession number: A7Z6E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program