A7Z612 (AROA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	RBAM_020760

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325330

Sequences

	Sequence		Length	Mass (Da)
	A7Z612 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 6653F2DE40505AF2	FASTA	428	45,049
	10 20 30 40 50 60 MKREKVTSLN GEIHIPGDKS ISHRSVMFGA LAEGTTTVKN FLPGADCLST IACFRKMGVS 70 80 90 100 110 120 IEQNGSDVTI YGKGIDALCE PDSLLDVGNS GTTIRLMLGI LAGRPFHSTV AGDESIAKRP 130 140 150 160 170 180 MKRVTEPLKQ MGAAIDGRAD GGFTPLSVRG GQLKGIDYVS PVASAQIKSA VLLAGLQAEG 190 200 210 220 230 240 TTTVTEPHKS RDHTERMLNA FGAELAETET SASVTGGQKL RGADIFVPGD ISSAAFFLAA 250 260 270 280 290 300 GAVVPGSRIV LKNVGLNPTR TGMIDVLKQM GASLEVIPSE ADSAEPYGDL VIETSALKAA 310 320 330 340 350 360 EIGGEIIPRL IDEIPIIALL ATQAEGTTVI KDAAELKVKE TNRIDTVVSE LRKIGADIEP 370 380 390 400 410 420 TEDGMKIHGK KTLAGGADVS SHGDHRIGMM LGVASCLTEE PIDIRDTEAI HVSYPTFFEH LDQLAKKA

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74438.1.
RefSeq	YP_001421669.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z612.
SMR	A7Z612. Positions 3-425.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z612.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000004766; EBBACP00000004644; EBBACG00000004758.
GeneID	5460940.
GenomeReviews	Gene locus RBAM_020760 in contig CP000560_GR.
KEGG	bay:RBAM_020760.
PATRIC	18749232. VBIBacAmy31356_2092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
GeneTree	EBGT00050000001036.
HOGENOM	HBG646626.
OMA	LECANIS.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_020760-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BACA2

Accession

Primary (citable) accession number: A7Z612

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program