Reviewed, UniProtKB/Swiss-Prot A7Z5Y7 (SYN_BACA2)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	RBAM_020510

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051376

Sequences

	Sequence		Length	Mass (Da)
	A7Z5Y7-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: A2F54FCFCFE3B173	FASTA	430	49,108
	10 20 30 40 50 60 MKTTINQVYK HTGEEVMIGA WVANKRSSGK IAFLQLRDGT GFIQGVVVKA EVEEDIFQIA 70 80 90 100 110 120 KSVTQETSLY VKGIVKEDER SPLGYELAVT SIEVIHEATD YPITPKEHGT EFLMDHRHLW 130 140 150 160 170 180 LRSKRQHAIM KIRNEIIRAT YEFFNKEGFV KVDPPILTGS APEGTTELFA TKYFDEDAFL 190 200 210 220 230 240 SQSGQLYMEA AAMALGKVFS FGPTFRAEKS KTKRHLIEFW MIEPEMAFVE FEENLEVQEN 250 260 270 280 290 300 YVAYIVQSVL EHCKIELNTL GRDTSKLEQI KAPFPRITYD KAIEFLKEKG FDDIEWGDDF 310 320 330 340 350 360 GAPHETAIAE SYDKPVFITH YPTSLKPFYM QPAKDRDDVV LCADLIAPEG YGEIIGGSER 370 380 390 400 410 420 VHDMDLLEER LKEHGLDSDA YKWYAELRQY GSVPHSGFGL GLERTVAWIS GAPHVRETIP 430 FPRLLNRLYP

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74413.1.
RefSeq	YP_001421644.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z5Y7.
Genome annotation databases
GeneID	5462020.
GenomeReviews	Gene locus RBAM_020510 in contig CP000560_GR.
KEGG	bay:RBAM_020510.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACA2

Accession

Primary (citable) accession number: A7Z5Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)