Reviewed, UniProtKB/Swiss-Prot A7Z5T7 (ILVD_BACA2)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	RBAM_020010

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000956
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z5T7-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 0368776773C62597	FASTA	558	59,404
	10 20 30 40 50 60 MAELRSNMIT QGIDRAPHRS LLRAAGVKEE DFGKPFIAVC NSYIDIVPGH VHLQEFGKIV 70 80 90 100 110 120 KEAIREAGGV PFEFNTIGVD DGIAMGHIGM RYSLPSREII ADSVETVVSA HWFDGMVCIP 130 140 150 160 170 180 NCDKITPGML MAAMRINIPT IFVSGGPMAA GRTSDGRKIS LSSVFEGVGA YQAGKIGESD 190 200 210 220 230 240 LQELEQFGCP TCGSCSGMFT ANSMNCLSEA LGLALPGNGT ILATSPERRE FVRKSAAQLM 250 260 270 280 290 300 ETIKKDIKPR DIVTEKAIDN AFALDMALGG STNTVLHTLA LANEAGVEYS LERINEVAER 310 320 330 340 350 360 VPHLSKLAPA SDVFIEDLHE AGGVSAALNE LAKKEGALHL DALTVTGRTL GETIAGHEVK 370 380 390 400 410 420 DYDVIHPLDN PFTEKGGLAV LFGNLAPDGA IIKTGGVQDG ITRHEGPAVV FDSQDAALEG 430 440 450 460 470 480 IINRKVKEGD VVIIRYEGPK GGPGMPEMLA PTSQIVGMGL GPKVALITDG RFSGASRGLS 490 500 510 520 530 540 IGHVSPEAAE GGPLAFVENG DHVIVDIEKR ILDVQVPEEE WEKRKAEWKG FEPKVKTGYL 550 ARYSKLVTSA NTGGIMKI

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74363.1.
RefSeq	YP_001421594.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z5T7.
Genome annotation databases
GeneID	5459548.
GenomeReviews	Gene locus RBAM_020010 in contig CP000560_GR.
KEGG	bay:RBAM_020010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACA2

Accession

Primary (citable) accession number: A7Z5T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)