A7Z5J9 (ODO1_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoglutarate dehydrogenase E1 component
EC=1.2.4.2

Alternative name(s):
Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	RBAM_019130

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	944 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity. HAMAP MF_01169
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2. HAMAP MF_01169
Cofactor	Thiamine pyrophosphate By similarity. HAMAP MF_01169
Subunit structure	Homodimer By similarity. HAMAP MF_01169
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 944	944	2-oxoglutarate dehydrogenase E1 component HAMAP MF_01169		PRO_1000065696

Sequences

	Sequence		Length	Mass (Da)
	A7Z5J9 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 936E7315F09C54CD	FASTA	944	106,267
	10 20 30 40 50 60 MFQNNMKQRM NWEDFYGPNL GYALELYDQY AEDPDSIDPD LKDMFDELGA PPSQIKEASG 70 80 90 100 110 120 TKEQGRVTAD LIQKIAAAVK LAEDIRTYGH LNASVNPLRK DPKKSELFPL SDYGLTEEEM 130 140 150 160 170 180 KAIPASVICK DAPANITNGL EAIQHLRNTY KRTISFEFDH VHDFEERAWI TKMVESGELF 190 200 210 220 230 240 RKNSPEKLSA VLERLTEVEG FEQFLHRTFV GQKRFSIEGL DALVPVLDDI IAQSVKAGTT 250 260 270 280 290 300 NVNIGMAHRG RLNVLAHVLG KPYEIIFSEF QHAPNKDLVP SEGSTGISYG WTGDVKYHLG 310 320 330 340 350 360 ADRQLQDAET KSARITLANN PSHLEFINPI VEGSTRAAQE TRTQKGYPVQ DETKSLAILI 370 380 390 400 410 420 HGDAAFPGEG IVAETLNLSS LKGYQVGGAI HIIANNMIGF TTESDESRST KYASDLAKGY 430 440 450 460 470 480 EIPIVHVNAD DPEACLSAVK FAVEYRKRFN KDFLIDLIGY RRYGHNEMDE PSTTQPMLYD 490 500 510 520 530 540 AVRKHPTVKR IFAEKLVSEG LISEEKAQNI ETAVTKRIED AYKKVPAKKE DAVREIELPE 550 560 570 580 590 600 PVSNGFPDVD TAIDFDVLRK LNGELINWPE SFNVFGKLKR ILERRAKAFD DDRKVEWSLA 610 620 630 640 650 660 ESLAFASILK DGTPIRLTGQ DSERGTFAQR NLVLHDSETG EEFVALHHLD DCAASFTVHN 670 680 690 700 710 720 SPLSEGSVLG FEYGYNVYSP ETLVMWEAQY GDFANAAQVY FDQFISAGRA KWGQKSGLVM 730 740 750 760 770 780 LLPHGYEGQG PEHSSGRVER FLQLAAENNW TVANLTSAAQ YFHILRRQAK MLLREEIRPL 790 800 810 820 830 840 VIMTPKSLLR NPNTVSEVQE LSESRFKPVY EQSGLSHAYE KVTRVVLSSG KVSIDISDHF 850 860 870 880 890 900 NKMEGDKDWL HIARIEQLYP FPAKDTKELF AKLPNLQEIV WVQEEPQNMG AWSYISPYLS 910 920 930 940 EIAPKGVNVQ YIGRRRRSSP AEGDPTVHKK EQERIVSDSL TRKN

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74275.1.
RefSeq	YP_001421506.1. NC_009725.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z5J9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007771; EBBACP00000007649; EBBACG00000007763.
GeneID	5461918.
GenomeReviews	Gene locus RBAM_019130 in contig CP000560_GR.
KEGG	bay:RBAM_019130.
PATRIC	18748902. VBIBacAmy31356_1926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0567.
GeneTree	EBGT00050000001072.
HOGENOM	HBG289950.
OMA	EGDEPAF.
ProtClustDB	PRK09404.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_019130-MONOMER.
Family and domain databases
HAMAP	MF_01169. SucA_OdhA. [Tree]
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR023784. 2oxoglutarate_DH_E1_bac. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
KO	K00164.
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_BACA2

Accession

Primary (citable) accession number: A7Z5J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program