Reviewed, UniProtKB/Swiss-Prot A7Z5B3 (BIOD_BACA2)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	RBAM_018260

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000019548
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z5B3-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 35AA3F29E6E77F17	FASTA	233	25,540
	10 20 30 40 50 60 MRSFFVTGTD TGVGKTIVSC GLAAAFRQKQ ADIGVFKPFL SGVPRTHPES DTSLLKDMSQ 70 80 90 100 110 120 TALSHEEITP FEWKEPLAPY TAAMLEGKSV SMADVMAHWS KIKNRHECFI VEGAGGISVP 130 140 150 160 170 180 LGENYLVSDL IKAMELPAVI VTRPDLGTIN HTYLTVEYAK NMGIEVLGII INGVRSRPGL 190 200 210 220 230 DEKTNPSMIE TLCKVPILGI TPKLDHVSSE NIQHMIEKHI DVSSFMNLTQ MGR

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74189.1.
RefSeq	YP_001421420.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z5B3.
Genome annotation databases
GeneID	5459522.
GenomeReviews	Gene locus RBAM_018260 in contig CP000560_GR.
KEGG	bay:RBAM_018260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CLIEGAG.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACA2

Accession

Primary (citable) accession number: A7Z5B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)