A7Z5B3 (BIOD_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	RBAM_018260

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000019548
Regions
	Nucleotide binding	112 – 115	4	ATP By similarity
	Nucleotide binding	202 – 204	3	ATP By similarity
Sites
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	51	1	Magnesium 2 By similarity
	Metal binding	112	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	51	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z5B3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 35AA3F29E6E77F17	FASTA	233	25,540
	10 20 30 40 50 60 MRSFFVTGTD TGVGKTIVSC GLAAAFRQKQ ADIGVFKPFL SGVPRTHPES DTSLLKDMSQ 70 80 90 100 110 120 TALSHEEITP FEWKEPLAPY TAAMLEGKSV SMADVMAHWS KIKNRHECFI VEGAGGISVP 130 140 150 160 170 180 LGENYLVSDL IKAMELPAVI VTRPDLGTIN HTYLTVEYAK NMGIEVLGII INGVRSRPGL 190 200 210 220 230 DEKTNPSMIE TLCKVPILGI TPKLDHVSSE NIQHMIEKHI DVSSFMNLTQ MGR

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74189.1.
RefSeq	YP_001421420.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z5B3.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z5B3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006893; EBBACP00000006771; EBBACG00000006885.
GeneID	5459522.
GenomeReviews	Gene locus RBAM_018260 in contig CP000560_GR.
KEGG	bay:RBAM_018260.
PATRIC	18748726. VBIBacAmy31356_1839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
GeneTree	EBGT00050000001962.
HOGENOM	HBG650065.
OMA	PQMERIE.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_018260-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACA2

Accession

Primary (citable) accession number: A7Z5B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program