A7Z552 (LEXA_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	RBAM_017650

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	LexA repressor HAMAP MF_00015		PRO_1000001258
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	128	1	For autocatalytic cleavage activity By similarity
	Active site	166	1	For autocatalytic cleavage activity By similarity
	Site	92 – 93	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z552 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: D554A9D2D3BCDFDA	FASTA	206	23,077
	10 20 30 40 50 60 MTKLSKRQLD ILRFIKEEVK TKGYPPSVRE IGEAVGLASS STVHGHLARL ETKGLIRRDP 70 80 90 100 110 120 TKPRAIEVLD EEEVQIPKSQ VVNVPVIGKV TAGIPITAVE NIDEYFPLPD RMVPPGEHVF 130 140 150 160 170 180 MLEIMGESMI DAGIFDKDYV IVKQQNTANN GEIVVAMTED DEATVKRFYK EDNYVRLQPE 190 200 NPTMEPIILQ NVSILGKVIG VFRTVH

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74128.1.
RefSeq	YP_001421359.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z552.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z552.
Protein family/group databases
MEROPS	S24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000005687; EBBACP00000005565; EBBACG00000005679.
GeneID	5461606.
GenomeReviews	Gene locus RBAM_017650 in contig CP000560_GR.
KEGG	bay:RBAM_017650.
PATRIC	18748600. VBIBacAmy31356_1776.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
GeneTree	EBGT00050000002279.
HOGENOM	HBG679610.
OMA	IAENPDF.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_017650-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01356.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BACA2

Accession

Primary (citable) accession number: A7Z552

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program