Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:RBAM_017130
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000205631 – 314tRNA dimethylallyltransferaseAdd BLAST314

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei104Interaction with substrate tRNAUniRule annotation1
Sitei127Interaction with substrate tRNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliA7Z500
SMRiA7Z500
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 20Substrate bindingUniRule annotation6
Regioni38 – 41Interaction with substrate tRNAUniRule annotation4

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX Bacteria
COG0324 LUCA
HOGENOMiHOG000039995
KOiK00791
OMAiGTGLYIR

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR018022 IPT
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequencei

Sequence statusi: Complete.

A7Z500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNKKQPVVI LVGPTAVGKT KLSIGLAKML NAEIISGDSM QVYKGMDIGT
60 70 80 90 100
AKVTEEETEG VPHHLIDILE PSDTFSTADY QKMVRGKITE IADRGKLPMI
110 120 130 140 150
VGGTGLYIQS VLYDYTFTEN SGDPVFRERM EKLAAEEGAE ALHAKLAAAD
160 170 180 190 200
PEAAAAIHQN NTRRVIRALE ILHVSGKTMS EHLKTQKREL LYNAVLIGLT
210 220 230 240 250
MDREVLYGRI NERVDLMLET GLLDEVKRLY DMNIRDSQSI QAIGYKELYE
260 270 280 290 300
YLDGKVPLPQ AVEQLKQNSR RYAKRQLTWF RNKMPVSWTD MTPPVDIEQK
310
KKEIFECIAG KLEI
Length:314
Mass (Da):35,397
Last modified:October 23, 2007 - v1
Checksum:i1ABFB01A7F6D7062
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA Translation: ABS74076.1
RefSeqiWP_007410375.1, NC_009725.1

Genome annotation databases

EnsemblBacteriaiABS74076; ABS74076; RBAM_017130
KEGGibay:RBAM_017130

Similar proteinsi

Entry informationi

Entry nameiMIAA_BACVZ
AccessioniPrimary (citable) accession number: A7Z500
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: April 25, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health