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Protein

Polyketide biosynthesis protein BaeE

Gene

baeE

Organism
Bacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductase activity.1 Publication

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851By similarity
Active sitei191 – 1911By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciBAMY326423:GCM4-1691-MONOMER.
UniPathwayiUPA01003.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyketide biosynthesis protein BaeE
Including the following 1 domains:
Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
Short name:
MCT
Gene namesi
Name:baeE
Ordered Locus Names:RBAM_016930
OrganismiBacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001120 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746Polyketide biosynthesis protein BaeEPRO_0000388001Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326423.RBAM_016930.

Structurei

3D structure databases

ProteinModelPortaliA7Z4Y0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 312312Acyl transferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FabD family.Curated

Phylogenomic databases

eggNOGiCOG0331.
HOGENOMiHOG000275975.
KOiK15329.
OMAiGGMYRGI.
OrthoDBiEOG67X1P6.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.366.10. 2 hits.
InterProiIPR004136. 2Npropane_dOase.
IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013785. Aldolase_TIM.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR014179. PfaD_fam.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF03060. NMO. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsiTIGR00128. fabD. 1 hit.
TIGR02814. pfaD_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

A7Z4Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISFVFPGQG SQRIGMGEDL FGRYPELTAK ADHILGYSIQ ELCRDGERLN
60 70 80 90 100
QTQFTQPALY VVNALSYLKK TEETGLKPDF TAGHSLGEYN ALYASGAFDF
110 120 130 140 150
EEGLQLVKKR GELMSRAKGG GMAAVIGLTH EQVTDVLREN HLDMIDIANM
160 170 180 190 200
NTPQQIVISG YKEDIEKAAS VFEAVNGVKM VHRLNVSGAF HSRYMLEAKE
210 220 230 240 250
EFSRFIESFH FKPLSIPVIS NVTARPYEQR ELKETLTGQI TGSVNWTDSI
260 270 280 290 300
RFLMGRKNMS FEEIGPGKVL TGLIQRITAE AEPITDEIKV PAEAGKSSIT
310 320 330 340 350
AASLGNEEFK RDYQLKYAYL AGGMYRGISS KEMVVKLAEK GMMGFFGTGG
360 370 380 390 400
LNIAHVEDAI LSIQHELRDG GSFGINVVHN MKHTDSEEKM IDLLLKHGIQ
410 420 430 440 450
NLEASAFLTV TPALVRFRAK GLKRGAGGQI IARQRIIAKL SRPEVAEAFL
460 470 480 490 500
SPAPDHILQK LAAENKITAE EASLMREIPV AHDICVEADS GGHTDGGVAY
510 520 530 540 550
SLMPAIIRLR DDMMKKYRYG KTVRIGAAGG IGTPEAAMAA FMLGADFIVT
560 570 580 590 600
GSINQCTVEA ATSGLVKDLL QQMNVQDTAY APAGDMFESG SKVQVLKKGL
610 620 630 640 650
FFPTRASKLH ELYQRHRSIE EIDEKTLRQI EEKYFKASVS SIYDKVKAHY
660 670 680 690 700
SNEDISKAER NPKEKMALIF KWYFRQSSAS AIKGDPDAKV DFQIHCGPAL
710 720 730 740
GAFNQWVKGT ELESWKNRHA DGIGMRLMEE TASLLNQKLG SFLQTC
Length:746
Mass (Da):82,426
Last modified:October 23, 2007 - v1
Checksum:iDCD623E4D0531171
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA. Translation: ABS74056.1.
RefSeqiWP_012117591.1. NC_009725.1.
YP_001421287.1. NC_009725.1.

Genome annotation databases

EnsemblBacteriaiABS74056; ABS74056; RBAM_016930.
KEGGibay:RBAM_016930.
PATRICi18748456. VBIBacAmy31356_1704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA. Translation: ABS74056.1.
RefSeqiWP_012117591.1. NC_009725.1.
YP_001421287.1. NC_009725.1.

3D structure databases

ProteinModelPortaliA7Z4Y0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326423.RBAM_016930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS74056; ABS74056; RBAM_016930.
KEGGibay:RBAM_016930.
PATRICi18748456. VBIBacAmy31356_1704.

Phylogenomic databases

eggNOGiCOG0331.
HOGENOMiHOG000275975.
KOiK15329.
OMAiGGMYRGI.
OrthoDBiEOG67X1P6.

Enzyme and pathway databases

UniPathwayiUPA01003.
BioCyciBAMY326423:GCM4-1691-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.366.10. 2 hits.
InterProiIPR004136. 2Npropane_dOase.
IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013785. Aldolase_TIM.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR014179. PfaD_fam.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF03060. NMO. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsiTIGR00128. fabD. 1 hit.
TIGR02814. pfaD_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 23117 / BGSC 10A6 / FZB42.
  2. "Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42."
    Chen X.-H., Vater J., Piel J., Franke P., Scholz R., Schneider K., Koumoutsi A., Hitzeroth G., Grammel N., Strittmatter A.W., Gottschalk G., Suessmuth R.D., Borriss R.
    J. Bacteriol. 188:4024-4036(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PATHWAY, FUNCTION IN BACILLAENE BIOSYNTHESIS.

Entry informationi

Entry nameiBAEE_BACA2
AccessioniPrimary (citable) accession number: A7Z4Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: October 23, 2007
Last modified: April 1, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.