A7Z4X1 (BIOF1_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
8-amino-7-oxononanoate synthase 1
Short name=AONS
EC=2.3.1.47

Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
Short name=KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase

Gene names

Ordered Locus Names:

RBAM_016840

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus amyloliquefaciens › Bacillus amyloliquefaciens subsp. plantarum

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide By similarity.
Catalytic activity	Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	8-amino-7-oxononanoate synthase activity Inferred from sequence or structural similarity. Source: UniProtKB glycine C-acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB pyridoxal phosphate binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	8-amino-7-oxononanoate synthase 1		PRO_0000380903
Regions
	Region	108 – 109	2	Pyridoxal phosphate binding By similarity
	Region	205 – 208	4	Pyridoxal phosphate binding By similarity
	Region	236 – 239	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	133	1	Substrate By similarity
	Binding site	180	1	Pyridoxal phosphate By similarity
	Binding site	353	1	Substrate By similarity
Amino acid modifications
	Modified residue	239	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z4X1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: B0A106263B61C6C0	FASTA	391	43,176
	10 20 30 40 50 60 MKEFEFLKAE LDKMKENKTW QQMKQIETKQ GPSVEVKGEN VIQLSSNNYL GLTSHPRLVE 70 80 90 100 110 120 AAKRAAEEFG AGTGSVRTIA GTFTMHNELE KKLANFKKTE AALVFQSGFT TNQGVLSSIL 130 140 150 160 170 180 TKEDIVISDE LNHASIIDGI RLTKADKKVY RHVDMDDLER VLKKSMNYRM RLIVTDGVFS 190 200 210 220 230 240 MDGNIAPLPD IVKLAEAYDA FVMVDDAHAS GVLGKNGRGT VNHFGLDGRV HIQVGTLSKA 250 260 270 280 290 300 IGVLGGYAAG SQVLIDYLRH KGRPFLFSTS HPPAVTAACI EAIDVLLEEP EHMEKLWENT 310 320 330 340 350 360 AYFKDKLVQM GLTLTKSETP IVPILIGEEE KAQALSDLLL TRGVFAQSIV YPTVAQGKAR 370 380 390 IRTIITAEHT NEELDRALEV IRSAAKELQL V

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74047.1.
RefSeq	YP_001421278.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z4X1.
ModBase	Search...
Protein-protein interaction databases
STRING	326423.RBAM_016840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS74047; ABS74047; RBAM_016840.
GeneID	5462068.
KEGG	bay:RBAM_016840.
PATRIC	18748436. VBIBacAmy31356_1694.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0156.
HOGENOM	HOG000221022.
KO	K00639.
OMA	VTAACIE.
ProtClustDB	PRK06939.
Enzyme and pathway databases
BioCyc	BAMY326423:GCM4-1682-MONOMER.
UniPathway	UPA00078.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR004723. BioF. IPR010962. PyrdxlP-dep_AcTrfase_put. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00858. bioF. 1 hit. TIGR01825. gly_Cac_T_rel. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIOF1_BACA2

Accession

Primary (citable) accession number: A7Z4X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	October 23, 2007
Last modified:	May 29, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program