A7Z4S8 (SYP_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	RBAM_016410

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Proline--tRNA ligase HAMAP MF_01569		PRO_1000069120

Sequences

	Sequence		Length	Mass (Da)
	A7Z4S8 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: CCED5064FD23FA84	FASTA	564	63,123
	10 20 30 40 50 60 MRQSLTLIPT LREVPADAEA KSHKLLLRAG FIRQNTSGVY SYMPLAYRVI QNIQKIVREE 70 80 90 100 110 120 MEKINAVEML MPALQQAETW QESGRWYTYG PELMRLKDRH GREFALGATH EEVITSLVRD 130 140 150 160 170 180 EVKSYKRLPL TLYQIQSKFR DEKRPRFGLL RGREFIMKDA YSFHASEESL EETYQNMYGA 190 200 210 220 230 240 YSSIFARCEL NVRPVIADSG AMGGKDTHEF MALSEIGEDT IAYSDGSSYA ANTEMAEVVM 250 260 270 280 290 300 NSEPSDETPE ALEKIDTPNV KSIEELASFL QIEPSACIKS MLFKADDRFV LVLVRGDHEV 310 320 330 340 350 360 NDVKVKNLLN AEVVELASHE EVAEKLGTEP GFAGPIGAAG DIEIYADQAV KVMVNAVSGA 370 380 390 400 410 420 NEKDRHYRNV NIERDASVKA YADLRIIQEG DPSPDGKGTI RFAEGIEVGQ VFKLGTRYSE 430 440 450 460 470 480 AMNATYLDEN GRAQPMLMGC YGIGVSRTLS AIAEQHHDEK GLIWPKSVAP YDLHILALNM 490 500 510 520 530 540 KNEAQKELAE QLYGKFQAEG YEVLYDDRAE RAGVKFADSD LIGLPIRITV GKRADEGIVE 550 560 VKIRKTGESA EVSVDELSEF IKTK

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS74004.1.
RefSeq	YP_001421235.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z4S8.
SMR	A7Z4S8. Positions 1-557.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006446; EBBACP00000006324; EBBACG00000006438.
GeneID	5461385.
GenomeReviews	Gene locus RBAM_016410 in contig CP000560_GR.
KEGG	bay:RBAM_016410.
PATRIC	18748348. VBIBacAmy31356_1650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000001167.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_016410-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACA2

Accession

Primary (citable) accession number: A7Z4S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program