Reviewed, UniProtKB/Swiss-Prot A7Z4S6 (DXR_BACA2)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	RBAM_016390

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000020216
Regions
	Nucleotide binding	7 – 36	30	NADP By similarity
Sites
	Metal binding	148	1	Divalent metal cation By similarity
	Metal binding	150	1	Divalent metal cation By similarity
	Metal binding	219	1	Divalent metal cation By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	219	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z4S6-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 1A20AD3C76AEFF44	FASTA	383	42,452
	10 20 30 40 50 60 MKNICLLGAT GSIGEQTLDV LRMHQDQFRL VSMTFGKNAE KAIPLIRTFQ PKYIAVGDMD 70 80 90 100 110 120 TYQKVKEASF SHECQIGIGE EGLIEAAVMD EVDIVVNALL GSVGLIPTLK AIEQKKTIAL 130 140 150 160 170 180 ANKETLVTAG HIVKGHAKRY GVPLLPVDSE HSAIFQALQG EQCKNIERLV ITASGGSFRD 190 200 210 220 230 240 KTRQELESVT IADALNHPNW SMGAKITIDS ATMMNKGLEV IEAHWLFDIP YEQIDVVLHK 250 260 270 280 290 300 ESIIHSMVEF HDRSVIAQLG NPDMRVPIQY ALTYPDRLPL PETKRLELWE IGSLHFAKAD 310 320 330 340 350 360 FERFRCLQFA FESGKIGGTM PTVLNAANEE AVAAFLAGRI SFLSIEDLIE QALDRHSVIQ 370 380 DPSLADIQEV DKDTRGYVNS ILT

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS74002.1.
RefSeq	YP_001421233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4S6.
Genome annotation databases
GeneID	5461383.
GenomeReviews	Gene locus RBAM_016390 in contig CP000560_GR.
KEGG	bay:RBAM_016390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IHSMVEY.
Family and domain databases
HAMAP	MF_00183. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_BACA2

Accession

Primary (citable) accession number: A7Z4S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)