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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:RBAM_015960
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000729761 – 435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST435

Structurei

3D structure databases

ProteinModelPortaliA7Z4N3.
SMRiA7Z4N3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
PANTHERiPTHR11806. PTHR11806. 1 hit.
PTHR11806:SF5. PTHR11806:SF5. 1 hit.
PfamiView protein in Pfam
PF01134. GIDA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiView protein in PROSITE
PS01281. GIDA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A7Z4N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQQTVNVIG AGLAGSEAAW QLAKRGIQVK LYEMRPVRQT AAHHTDKFAE
60 70 80 90 100
LVCSNSLRSN TLANAVGVLK EEMRALDSAI IQSADKCSVP AGGALAVDRH
110 120 130 140 150
EFAQSVTDLV KNHPNVTVLT EEVTEIPEGP TVIATGPLTS ESLSQQLKEL
160 170 180 190 200
TGEEYLYFYD AAAPIVEKDS LDMEKVYLKS RYDKGEAAYL NCPMTEEEFD
210 220 230 240 250
RFYEALTTAE TVPLKEFEKE IFFEGCMPIE VMAKRGKKTM LFGPMKPVGL
260 270 280 290 300
EDPKTGKRPY AVVQLRQDDA AGTLYNIVGF QTHLKWGDQK EVLKLIPGLE
310 320 330 340 350
NVDIVRYGVM HRNTFINSPS LLNPTYQFKQ RNDLFFAGQM TGVEGYVESA
360 370 380 390 400
ASGLVAGINA AKLVLGQDLV TFPQETAIGS MAHYITTTNQ KNFQPMNANF
410 420 430
GLLKELPVKI KNKKERNEEY AKRAIETIQT ISKTI
Length:435
Mass (Da):48,258
Last modified:October 23, 2007 - v1
Checksum:i3F3E057B80892FFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA. Translation: ABS73959.1.
RefSeqiWP_012117550.1. NC_009725.1.

Genome annotation databases

EnsemblBacteriaiABS73959; ABS73959; RBAM_015960.
KEGGibay:RBAM_015960.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMFO_BACVZ
AccessioniPrimary (citable) accession number: A7Z4N3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 23, 2007
Last modified: July 5, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families