A7Z4N2 (A7Z4N2_BACA2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL ABS73958.1
Ordered Locus Names:	RBAM_015950

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A7Z4N2 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F242F7BEE53C6D40	FASTA	691	79,081
	10 20 30 40 50 60 MSDYLVIVES PAKAKTIERY LGKKYKVKAS MGHVRDLPKS QMGVDIEQNF EPKYITIRGK 70 80 90 100 110 120 GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL DLSSDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRMINM DLVDAQQARR ILDRLVGYKI SPILWKKVKK GLSAGRVQSV ALRLIIDREK 190 200 210 220 230 240 EINDFKPEEY WTITGSFLKG KETFEAGFFG KNGKKLPLKS EEDVKAILAQ LKGNKYTVDK 250 260 270 280 290 300 VTKKERKRNP ALPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGKE GTVGLITYMR 310 320 330 340 350 360 TDSTRISNTA VEEASAFIDQ AYGKEYLAGK RKPAKKNENA QDAHEAIRPT SVLRKPADLK 370 380 390 400 410 420 AVLGRDQLRL YKLIWERFVA SQMAPAVLDT MSVDLSNNGL TFRANGSKVK FAGFMKVYVE 430 440 450 460 470 480 GKDDQLEEKD RMLPDLKEGD TVLSKDIEPE QHFTQPPPRY TEARLVKTLE ELGIGRPSTY 490 500 510 520 530 540 APTLDTIQRR GYVGLDNKRF VPTELGQIVL DLIMEFFPEI INVEFTAKME RDLDHVEDGQ 550 560 570 580 590 600 TEWVQIIDSF YTDFEKRVKK AEAEMKEVEI EPEYADEDCE LCGSRMVYKM GRYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKQIGVKCP KCHEGNIVER KSKKKRIFYG CDRYPECDFV SWDKPIERKC 670 680 690 PKCENMLVEK KLKKGIQVQC VECDYKEEPQ K

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73958.1.
RefSeq	YP_001421189.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z4N2.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000005182; EBBACP00000005060; EBBACG00000005174.
GeneID	5463280.
GenomeReviews	Gene locus RBAM_015950 in contig CP000560_GR.
KEGG	bay:RBAM_015950.
PATRIC	18748256. VBIBacAmy31356_1604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A7Z4N2_BACA2

Accession

Primary (citable) accession number: A7Z4N2

Entry history

Integrated into UniProtKB/TrEMBL:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)